Nilai Diagnostik Pemeriksaan Reaksi Rantai Polimerase pada Tuberkulosis Paru Sputum Basil Tahan Asam Negatif

Putra, I Wayan Agus and Surjanto, Eddy and Suradi, and Aditama, Tjandra Yoga (2008) Nilai Diagnostik Pemeriksaan Reaksi Rantai Polimerase pada Tuberkulosis Paru Sputum Basil Tahan Asam Negatif. J Respir Indo, 28 (3). pp. 136-144.

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    Abstract

    Tuberculosis (TB) temains an important health problem in Indonesia. Diagnosis of pulmonary tuberculosis requires sputum examination and isolation of M, Tuberculosis from a culture specimen, The limitation of acid-fast bacili (AFB) microscopic has relatively low sensitivity and solid media culture of M. tuberculosis required for long time (4-6 weeks) in indentifying M. tuberculosis. Approximately 30-60% of pulmonary tuberculosis recorded negative AFB. Thus patients with negative AFB were usually clinical and unspecified radiological. Polymerase Chain Reaction (PCR) method amplifying DNA or RNA in vitro were the method used as dection, indentification and sensitivity test of Mycobacterium tuberculosis. The aim of this study was to determine the validity of PCR for the diagnosis pulmonary tuberculosisnegative AFB and to obtain the method more rapid for detection of M. tuberculosis that than solid media Kudoh. A cross sectional study was conducted in Moewardi General Hospital Surakarta. Seventy four patients of pulmonary TB with negative AFB were reruited in this study. All sputum specimens were examined on the solid media (Kudoh) and PCR method. All patients were given antituberculosis drug. Respond of the therapy would be analysed in the following 2 months. We got 26 patients positive culture media solid Kudoh and 61 patients positive PCR from the sample of 74 specimens with pulmonary tuberculosis negative AFB. The sensitivity and specification of PCR on the solid media Kudoh were 96% and 25%. PCR onclinical responds were 84.7% and 100%. The conclusion of this study were the sensitivity of PCR method for the diagnosis of pulmonary tuberculosis negative AFB relativity high (96%) but not specificity (25%). Key words : negative AFB microscopic, solid media culture, PCR, diagnostic value.

    Item Type: Article
    Subjects: R Medicine > R Medicine (General)
    Divisions: Fakultas Kedokteran > Pendidikan Kedokteran
    Depositing User: mr azis r
    Date Deposited: 27 May 2013 22:21
    Last Modified: 27 May 2013 22:21
    URI: https://eprints.uns.ac.id/id/eprint/908

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