Polimorfisme DNA pada Lokus-2 Gen Hormon Pertumbuhan Sapi Madura DNA polymorphism at locus-2 of growth hormone gene of Madura cattle

PURWOKO, AGUS and SUTARNO, and ETIKAWATI, NITA (2003) Polimorfisme DNA pada Lokus-2 Gen Hormon Pertumbuhan Sapi Madura DNA polymorphism at locus-2 of growth hormone gene of Madura cattle. BIODIVERSITAS, 4 (1). pp. 7-11. ISSN 1412-033X

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    Abstract

    The objectives of the research were to detect DNA polymorphism at locus 2 of bovine growth hormone gene of Madura cattle and to know its genetic diversity. DNA polymorphisms and their effect on phenotypic traits have been studied widely in dairy cattle but not for beef cattle, especially for Indonesian local cattle. Polymorphism was detected using PCR-RFLP using primer GH-5 and GH-6 for amplifying locus 2 of growth hormone gene. Genetic diversity was analyzed based on the formula of Nei (1973, 1975). DNA polymorphism was found on locus 2 of growth hormone gene using MspI restriction enzyme. This polymorphism may be caused the lost of restriction MspI site. The genetic diversity was 0.4422. Key words: bovine growth hormon gene, PCR-RFLP, polymorphism, Msp I restriction enzyme, Madura Cattle.

    Item Type: Article
    Subjects: Q Science > Q Science (General)
    Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam > Biologi
    Depositing User: mr azis r
    Date Deposited: 21 May 2013 19:19
    Last Modified: 21 May 2013 19:19
    URI: https://eprints.uns.ac.id/id/eprint/789

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