Isolati on and characterization of an AGAMOUS homologue from cocoa

Chaidamsari, Tetty and Samanhudi, and Sugiarti, Herti and Santoso, Djoko and Angenent, Gerco C. and de Maagd, Ruud A. (2006) Isolati on and characterization of an AGAMOUS homologue from cocoa. Plant Science (170). pp. 968-975.

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We report the cloning of a cDNA from TcAG,an AG (Arabidopsis thaliana MADS-box C-type transcription factor gene AGAMOUS ) homologue from cocoa ( Theobroma cacao L.). TcAG was in the cocoa ?ower expressed primarily in stamens and ovaries, comparable to AG in Arabidopsis. Additionally, we found that TcAG is also expressed in the fruit (pod) wall and during its entire development, as well as in the fruit pulp. Ectopic expression of TcAG in transgenic A. thaliana plants resulted in a range of weak to strong apetala2 (ap2 ) mutant-like phenotypes as well as early ?owering and curly leaves, as observed in other studies of plants overexpressing a functional AG homologue. The severity of the phenotypes correlated positively with the TcAG transcript level in the transgenic plants. Keywords: Flower development; Cocoa; AGAMOUS; MADS-box

Item Type: Article
Subjects: S Agriculture > S Agriculture (General)
S Agriculture > SB Plant culture
Depositing User: mr admin 007
Date Deposited: 20 May 2013 19:25
Last Modified: 20 May 2013 19:25

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