ANALISIS KERAGAMAN POLA PITA ISOZIM TANAMAN MANGGIS (Garcinia mangostana L.) JOGOROGO

KARTIKA NUGRAHA, DANY (2008) ANALISIS KERAGAMAN POLA PITA ISOZIM TANAMAN MANGGIS (Garcinia mangostana L.) JOGOROGO. Other thesis, Universitas Sebelas Maret.

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    Abstract

    Mangosteen ( Garcinia mangostana L.) is original plant fruit of Southeast Asia region, include Indonesia. In Indonesia, exactly at Jogorogo village, Ngawi's regency, East of Java province, there is one mangosteen plant ecotype which has several special characteristic such as : nice taste, little yellow gum, smooth fruit skin and fruit easy to open. The character and diversity of genetic of Jogorogo’s mangosteen not known yet, so genetic analisis by isozyme banding pattern diversity analysis is neecesseried. The research aim is to find out the isozyme banding pattern diversity of Jogorogo’s mangosteen. The research was conducted at MIPA'S Center Laboratory, University of Sebelas Maret Surakarta from September until October 2007. Starch gel horizontal type of electrophoresis was used in the analysis with four enzyme systems, there are peroxidase (PER), esterase (EST), acid phospatase (ACP) and aspartate aminotransferase (AAT). The observation of data as zimogram or isozyme’s bandings that is made in migration distance point (Rf). Migration distance point representating the non-similarity distance (euclidean) which continued by dendrogram analysis. Dendrogram analysis conducted by “ Hierarchical Cluster Analysis ” method with “ Average Linkage (Between Groups). The result of the research, the mangosteen Jogorogo (10 samples) has isozyme banding pattern diversity, there are : 5 banding patterns available on isozim peroxidase (PER), 6 banding patterns on isozim esterase (EST), 4 banding patterns on isozim acid phospatase (ACP) and 3 banding patterns on isozim aspartate aminotransferase (AAT). Dendrogram bases four enzyme systems (PER, EST, ACP and AAT) on non-similarity distance (euclidean) 0. 15 could be grouped into 4 clusters, where is first group consisting of 6 samples (number sample 2, 4, 1, 9, 7 and 5) having similarity genetic 85 %, that showed genetic relationship between its group member is close.Mangosteen ( Garcinia mangostana L.) is original plant fruit of Southeast Asia region, include Indonesia. In Indonesia, exactly at Jogorogo village, Ngawi's regency, East of Java province, there is one mangosteen plant ecotype which has several special characteristic such as : nice taste, little yellow gum, smooth fruit skin and fruit easy to open. The character and diversity of genetic of Jogorogo’s mangosteen not known yet, so genetic analisis by isozyme banding pattern diversity analysis is neecesseried. The research aim is to find out the isozyme banding pattern diversity of Jogorogo’s mangosteen. The research was conducted at MIPA'S Center Laboratory, University of Sebelas Maret Surakarta from September until October 2007. Starch gel horizontal type of electrophoresis was used in the analysis with four enzyme systems, there are peroxidase (PER), esterase (EST), acid phospatase (ACP) and aspartate aminotransferase (AAT). The observation of data as zimogram or isozyme’s bandings that is made in migration distance point (Rf). Migration distance point representating the non-similarity distance (euclidean) which continued by dendrogram analysis. Dendrogram analysis conducted by “ Hierarchical Cluster Analysis ” method with “ Average Linkage (Between Groups). The result of the research, the mangosteen Jogorogo (10 samples) has isozyme banding pattern diversity, there are : 5 banding patterns available on isozim peroxidase (PER), 6 banding patterns on isozim esterase (EST), 4 banding patterns on isozim acid phospatase (ACP) and 3 banding patterns on isozim aspartate aminotransferase (AAT). Dendrogram bases four enzyme systems (PER, EST, ACP and AAT) on non-similarity distance (euclidean) 0. 15 could be grouped into 4 clusters, where is first group consisting of 6 samples (number sample 2, 4, 1, 9, 7 and 5) having similarity genetic 85 %, that showed genetic relationship between its group member is close.

    Item Type: Thesis (Other)
    Subjects: G Geography. Anthropology. Recreation > GE Environmental Sciences
    S Agriculture > S Agriculture (General)
    S Agriculture > SB Plant culture
    Divisions: Fakultas Pertanian
    Depositing User: Users 837 not found.
    Date Deposited: 18 Jul 2013 17:52
    Last Modified: 18 Jul 2013 17:52
    URI: https://eprints.uns.ac.id/id/eprint/6572

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