PERTUMBUHAN DAN KANDUNGAN RESERPIN KULTUR KALUS Rauvolfia verticillata (Lour.) Baillon PADA VARIASI KONSENTRASI SUKROSA DALAM MEDIA MS

Irmawati, (2007) PERTUMBUHAN DAN KANDUNGAN RESERPIN KULTUR KALUS Rauvolfia verticillata (Lour.) Baillon PADA VARIASI KONSENTRASI SUKROSA DALAM MEDIA MS. Other thesis, UNIVERSITAS SEBELAS MARET.

[img] PDF - Published Version
Download (2745Kb)

    Abstract

    ABSTRACT Irmawati. 2006. THE GROWTH AND RESERPINE CONTENT OF CALLUS CULTURE OF Rauvolfia verticillata (Lour.) Baillon ON THE VARIATION OF SUCROSE CONCENTRATION IN MS MEDIUM. Department of Biology. Faculty of Mathematics and Natural Science. Sebelas Maret University. Surakarta. The aim of this research is to study the effect of variation of sucrose concentration on growth and reserpine content on callus culture of R. verticillata. The research was conducted with callus culture method that consist of two stages. The first stage is callus initiation to induce callus from explant (R. verticillata leaf), and the second stage is reserpine production on treatment medium. This research uses Complately Randomized Desaign (CRD) by one factor, variation of sucrose concentration. The sucrose concentration consists of five levels e. q 0 g/l, 10 g/l, 20 g/l, 30 g/l and 40 g/l, and each concentration in five replicates. The collected data consist of qualitative and quantitative data. The qualitative data (callus morphology) presented descriptively. The quantitative data (fresh weight callus, dry weight callus, reserpine content) were analysed using ANOVA and followed by DMRT 5% confident level. The result of research showed that variation of sucrose concentration influence fresh weight callus, dry weight callus and reserpine content. The increasing of sucrose concentration tended to raise callus growth, which could be seen from the fresh and dry weight callus. The highest fresh weight callus was found in medium with sucrose concentration 20 g/l, while the highest dry weight callus was found in medium with sucrose concentration 40 g/l. The increasing of sucrose concentration until 30 g/l raised reserpine content, but sucrose concentration over 30 g/l decreased reserpine content.

    Item Type: Thesis (Other)
    Subjects: Q Science > QH Natural history
    Q Science > QH Natural history > QH301 Biology
    Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam > Biologi
    Depositing User: Saputro Bagus
    Date Deposited: 15 Jul 2013 16:31
    Last Modified: 15 Jul 2013 16:31
    URI: https://eprints.uns.ac.id/id/eprint/4414

    Actions (login required)

    View Item