SKRINING FITOKIMIA DAN PROFIL KROMATOGRAFI LAPIS TIPIS EKSTRAK LUMUT DAUN Campylopus umbellatus (S. G.) Par.

FARDA, HUSNA (2019) SKRINING FITOKIMIA DAN PROFIL KROMATOGRAFI LAPIS TIPIS EKSTRAK LUMUT DAUN Campylopus umbellatus (S. G.) Par. Other thesis, Universitas Sebelas Maret.

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    Abstract

    Campylopus umbellatus (S. G.) Par. is one type of leaf moss plants are commonly found in Indonesia. Until now, information on the use of moss plants has not been reported yet. So it is necessary to conduct a research to identify the class of secondary metabolite composition in C. umbellatus extracts through the Thin Layer Chromatography (TLC) profile. The aims of this research were to find out the profile of Thin Layer Chromatography and group of secondary metabolites composition in C. umbellatus extracts. Extraction of C. umbellatus using the maceration method with Chloroform and ethanol 70%. Separation of secondary metabolites by Thin Layer Chromatography method with silica gel stationary phase GF254 and the mobile phase of chloroform: ethyl acetate (9: 1 v / v), ethanol: n-hexane (9: 1 v / v) and ethyl acetate (100%). Detection of secondary metabolite composition using Serium (IV) sulfate reagent, Dragendorff reagent, Lieberman Burchad reagent, FeCl3 reagent and vapor ammonia. Thin Layer Chromatography profile data and known types of chemical composition from spot sightings on TLC with specific reagents of certain compounds were analyzed descriptively. The results showed that C. umbellatus extract contain more nonpolar secondary metabolite compounds (alkaloids, steroids, terpenoids) with varying Rf values compared to polar ones (phenolic and flavonoid. From results, C. umbellatus extract contains alkaloid, steroids, terpenoids, flavonoids and phenolic compounds.

    Item Type: Thesis (Other)
    Subjects: Q Science > QH Natural history > QH301 Biology
    Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam
    Fakultas Matematika dan Ilmu Pengetahuan Alam > Biologi
    Depositing User: Ratri Dwi Saputri
    Date Deposited: 09 Jul 2019 10:07
    Last Modified: 09 Jul 2019 10:07
    URI: https://eprints.uns.ac.id/id/eprint/43967

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