perbedaan pengaruh pemberian ligan brusein-D dari fraksi etanolik buah makasar (brucea javanica (l.) MERR) dengan gemcitabine terhadap penghambatan pertumbuhan sel karsinoma pankreas PANC-1 dan ekspresi p53 mutan

HERNANTO, JODY (2018) perbedaan pengaruh pemberian ligan brusein-D dari fraksi etanolik buah makasar (brucea javanica (l.) MERR) dengan gemcitabine terhadap penghambatan pertumbuhan sel karsinoma pankreas PANC-1 dan ekspresi p53 mutan. Masters thesis, UNIVERSITAS SEBELAS MARET.

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    Abstract

    THE DIFFERENCE IN THE EFFECT OF BRUSEIN-D LIGAND FROM THE ETHANOLIC FRACTION OF THE MACASSAR FRUIT (Brucea Javanica (L.) Merr) WITH GEMCITABINE AGAINST INHIBITION OF CELL GROWTH OF PANC-1 PANCREATIC CANCER CELLS AND MUTANT P53 EXPRESSION Jody Hernanto, Ida Bagus Budhi Surya Adyana Digestive Surgery Division of, dr Moewardi General Hospital, Sebelas Maret University, Solo, Indonesia. Background Pancreatic carcinoma is highly invasive and resistant to conventional chemotherapy by producing solid stroma, rich in collagen fibers, extracellular matrix proteins, fibroblasts and inflammatory cells called desmoplastic reactions that form the barrier, so that pancreatic carcinoma becomes the most resistant cancer to chemotherapy. Objective In insilico, Brusein D (BD) ligand is highly toxic to pancreatic carcinoma cells compared to Gemcitabine (G). Research needs to be done to prove the ability of BD ligand to inhibit proliferation and induce apoptosis of pancreatic carcinoma cells and suppress mutant p53 protein expression in invitro. Methods A cytotoxicity test was performed on PANC-1 pancreatic cancer cells to search for LC50 from BD ligand and G. After LC50 was determined immunohistochemical test with concentration of half LC50, LC50 and twice LC50 then mutant p53 expression was shown in percentage of cells. Results LC50 of BD ligand is 28,86μl/ml and LC50 G is 1,455,55μg/ml. At the highest concentration of 20μl/ml BD ligand inhibited cancer cell proliferation 25.258%, while G at the highest concentration of 300 μg/ml was able to inhibit 2.06%. On the kruskal wallis statistic test p = 0,000 (p <0.05) means that there is a significant difference in G administration and BD ligand on mutant p53 expression. In the partial differential test it was found that the mean difference in the control group was higher than that of the other treatment groups in which the control group had different levels of mutant p53 expression, indicating that administration of G and BD ligand in the 2μg/ml and 1 μg/ml treatment was effective in suppressed the rate of mutant p53 expression in cultured PANC-1 pancreatic cancer cells, whereas at a dose of 0.5μg/ml both in G and BD was not effective in decreasing the expression level of mutant p53. Conclusions The potential inhibition of mutant p53 expression of PANC-1 pancreatic cancer cells by invading BD ligand shows a significant difference with G.

    Item Type: Thesis (Masters)
    Subjects: R Medicine > RD Surgery
    Divisions: Pascasarjana > Magister > Magister Kedokteran Keluarga
    Depositing User: Kuni Nur Aini
    Date Deposited: 27 Jul 2018 17:03
    Last Modified: 27 Jul 2018 17:03
    URI: https://eprints.uns.ac.id/id/eprint/41759

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