UJI ANATOMI, METABOLIT SEKUNDER, DAN MOLEKULER Sansevieria trifasciata

Febria Dewatisari. , Whika (2013) UJI ANATOMI, METABOLIT SEKUNDER, DAN MOLEKULER Sansevieria trifasciata. Masters thesis, Universitas Sebelas Maret Surakarta .

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    Abstract

    Sansevieria is an ornamental plant which has variation leaves on colour and form. It has unstabilizaton characteristic of genetic. This condition especially happens on species Sansevieria trifasciata. Sansevieria trifasciata which has been expressed to the colour and form of leaves. On the other hand S. trifasciata has an effect as medical plant. Its roots and leaves has many benefical of secondary metabolism such as saponins. Therefore diversity on S. trifasciata causing diversity on secondary metabolism like saponins. With knowing morphologhy variation in species S. trifasciata, we will know variation in content of saponin and isozyme banding pattern. The aim of this research was to look at different anatomycal structure for organs, the second was the experiment content of saponin as secondary metabolism, and third was to investigate any variation in their isozyme banding pattern of S. trifasciata “Green tiger”, “Hahnii medio picta”, “Green arrow”, “Golden hahnii”, serta “Hahnii cream”. The approach used were morphology and microscopic observation covering leaf, stalk, and root organs; isozyme banding pattern used electrophoresis method; and content of their saponin. Calculation the content of saponin, absorbantion was measured with Spectrophotometer, and then was conversed in mg/g. The genetic diversity was knowable from the analyze result of isozyme based on the matric of genetic distance which applied on the shape of dendogram with Hierarchical Cluster Analysis metode average linkage (between groups) program SPSS 14. The results showed that genetic diversity of S. trifasciata “Hahnii cream”, “Green arrow”, “Hahnii medio picta”, “Golden hahnii”, and “Green tiger” have been found. Morphology observations, there was a unique character on S. trifasciata “Hahnii medio picta”,while the microscopic observation on leaves organ, all variety have the same form, “Green arrow” has different on stalk cells compared with the others variety. On root cells of “Golden Hahnii” dan “Hahnii cream” they have same cell. From content of saponin, S. trifasciata “Golden hahnii” had the higest content among all, they were 1,7783 mg/g and 1,5810 mg/g. The dendogram resulting from peroxydase data S. trifasciata was clustered in two groups while S. trifasciata “Hahnii medio picta” has own character in group. Accordingly the esterase data also showing into two group, where S. trifasciata “Golden hahnii” has own character. Keywords : Sanseviera trifasciata,morphology, saponin, isozyme.

    Item Type: Thesis (Masters)
    Subjects: Q Science > Q Science (General)
    Divisions: Pasca Sarjana
    Pasca Sarjana > Magister > Biosains
    Depositing User: Riza Umami Nur K
    Date Deposited: 15 Jul 2013 02:26
    Last Modified: 15 Jul 2013 02:26
    URI: https://eprints.uns.ac.id/id/eprint/4159

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