PENGARUH PEMBERIAN JUS JAMBU BIJI MERAH (Psidium Guajava Linn.) TERHADAP KADAR ASAM URAT DAN KREATININ DARAH MENCIT PUTIH (Mus musculus) MODEL HIPERURISEMIA SEKUNDER

WD, APRILLINDA FITRI (2018) PENGARUH PEMBERIAN JUS JAMBU BIJI MERAH (Psidium Guajava Linn.) TERHADAP KADAR ASAM URAT DAN KREATININ DARAH MENCIT PUTIH (Mus musculus) MODEL HIPERURISEMIA SEKUNDER. Masters thesis, Universitas Sebelas Maret.

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    Abstract

    The effect of red guava juice (Psidium guajava Linn.) in decreasing uric acid and creatinine levels of hyperuricemic secondary white mice (Mus musculus) Aprillinda, F.W.1, Paramasari, D.2, Brian, W.3 1 Postgraduate Program of Nutrition Science, Universitas Sebelas Maret, Jl. Ir. Sutami 36A, Surakarta, Indonesia 2Faculty of Medicine, Universitas Sebelas Maret, Jl. Ir. Sutami 36A, Surakarta, Indonesia 3Faculty of Medicine, Universitas Sebelas Maret, Jl. Ir. Sutami 36A, Surakarta, Indonesia Corresponding: E-mail: april1211.af@gmail.com ABSTRACT Background: Uric acid is the final product of purine metabolism in humans. Increased uric acid production or decreased uric acid excretion can raise the blood creatinine level. Red guava (Psidium Guajava Linn.) contains a high amount of vitamin C, polyphenol, and flavonoids that serve as antioxidants. Empirically, red guava was used to cure various diseases, one of which is hyperuricemia secondary. Aim: This study aims to determine the effect of red guava juice on uric acid and creatinine levels of hyperuricemic secondarymodel in white mice (Mus musculus) Method: The present study used true experimental design with pretest-posttest control group method. Subjects were 48 two-months-old male, white mice BALB/C breed. The study subjects were categorized into 6 different groups, namely normal control (NC) group which were given standard feed; positive control (PC) group which were induced by potassium oxonate and were given standard feed; intervention 1 (I1) group which were induced by potassium oxonate, were given standard feed and allopurinol 0.26mg/Kg mice body weight/day; and intervention 2, 3, 4 (I2, I3, I4) groups which were induced by potassium oxonate, were given standard feed and red guava juice with the dose of 5 ml/Kg mice body weight/day, 10ml/Kg mice body weight/day, and 20ml/Kg mice body weight/day, respectively. The blood uric acid level of the mice was measured by an enzymatic method using FS TBHBA uric acid reagent. The blood creatinine level was measured by Jaffe method using Creatinine FS reagent. The data was then analyzed using One Way ANOVA and were considered significant if p-value < 0.05. Results: Based on statistical analysis, red guava juice with the dose of 5 ml/Kg mice body weight/day, 10 ml/Kg mice body weight/day, and 20 ml/Kg mice body weight/day can lower the uric acid level and creatinine level significantly (p < 0,05). The reductions in uric acid level dose 5 ml/Kg mice body weight/day were 4.90 ± 0.35 mg/dl (p=0.001), dose 10 ml/Kg mice body weight/day were 5.80 ± 0.39 mg/dl (p=0.001), dose 20 ml/Kg mice body weight/day were 6.71 ± 0.47 mg/dl (p=0.001). The reductions of blood creatinine level dose 5 ml/Kg mice body weight/day were 0.55 ± 0.07 mg/dl (p=0.001), dose 10 ml/Kg mice body weight/day were 1.67 ± 0.16 mg/dl (p=0.001), dose 20 ml/Kg mice body weight/day were 2.12 ± 0.14 mg/dl (p=0.001). Conclusion: The administration of red guava juice dose of 5 ml/Kg mice body weight/day, 10 ml/Kg mice body weight/day, and 20 ml/Kg mice body weight/day were significant can lower the uric acid and creatinine levels of mice (Mus musculus). The most effective dose on lowering uric acid and creatinine levels of hyperuricemic white mice (Mus musculus) was 20 ml/Kg mice body weight/day. Keywords: red guava (Psidium guajava linn.), uric acid, creatinine

    Item Type: Thesis (Masters)
    Subjects: Q Science > Q Science (General)
    R Medicine > RD Surgery
    Divisions: Pascasarjana
    Pascasarjana > Magister
    Pascasarjana > Magister > Ilmu Gizi
    Depositing User: Wahyu Trianingsih
    Date Deposited: 04 Jan 2018 00:18
    Last Modified: 04 Jan 2018 00:18
    URI: https://eprints.uns.ac.id/id/eprint/38864

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