PENGARUH PEMBERIAN L-ARGININE TERHADAP SEL ASTROSIT DAN BODY NEURON PADA OTAK MENCIT (Mus musculus) MODEL PREEKLAMPSIA

Ridwan, Robert (2017) PENGARUH PEMBERIAN L-ARGININE TERHADAP SEL ASTROSIT DAN BODY NEURON PADA OTAK MENCIT (Mus musculus) MODEL PREEKLAMPSIA. Masters thesis, Universitas Sebelas Maret.

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    Abstract

    Pengaruh L-Arginin Terhadap Sel Astrosit dan Body Neuron Pada Otak Mencit (Mus Musculus) Model Preeklampsia Robert Ridwan, Sri Sulistyowati, Supriyadi Hari R Departemen Obstetri Ginekologi Fakultas Kedokteran Universitas Sebelas Maret Rumah Sakit Umum Daerah Dr. Moewardi Surakarta ABSTRAK Latar Belakang: Preeklampsia menempati posisi tertinggi dalam kelompok gangguan hipertensi pada kehamilan dalam hal efek negatif terhadap kesehatan ibu maupun bayi. Ketidakseimbangan antara oxidant dan antioxidant yang menyebabkan stress oksidatif serta disfungsi endotel berperan pada pathogenesis preeklampsia. Peningkatan tekanan darah akut menyebabkan gangguan autoregulasi cerebrovaskuler, gangguan sawar darah otak, dan merusak protein dan serum konstituen di otak. Gangguan fungsi astrosit sebagai sawar darah otak dan pemeliharaan keseimbangan ion ekstraselular, serta apoptosis body neuron merupakan manifestasi gangguan autoregulasi cerebrovaskuler. L-Arginine mempunyai peran jalur L-arginin-nitric oxide pada preeklampsia berperan penting dalam hemodinamik sistemik dan regulasi radikal bebas. Penelitian ini bertujuan untuk membuktikan pengaruh pemberian L-Arginin terhadap sel astrosit dan body neuron pada mencit (Mus musculus) model preeklampsia. Metode: Penelitian eksperimental analitik ini dilakukan pada 30 mencit betina (Mus musculus) galur Swiss yang bunting enam belas hari, sehat, dengan berat badan 20-25 gram, untuk mengetahui perbedaan histopatologi sel astrosit dan body neuron pada mencit bunting normal (K(-)), mencit bunting model preeklampsia (K(+)) dan mencit bunting model preeklampsia (P) yang mendapat terapi L-Arginine. Pengambilan sampel preparat dilakukan pada otak mencit yang sebelumnya telah dibedah dan dilakukan blok parafin kemudian diberikan pewarnaan Hematoxylin Eosin (HE). Hasil dinilai secara semikuantitatif dengan mengukur jumlah sel pada tiap 5 lapang pandang. Analisis data menggunakan uji Oneway Anova and Post-Hoc dengan model Tukey test dengan SPSS (Software Package for social Science) 21. Hasil: Rerata sel astrosit K(-) 36.53±15.72, pada K(+) 24.84±8.70¬, dan pada (P) 30.46±9.23. Didapatkan perbedaan signifikan pada kelompok normal dengan kelompok preeklampsia (p=0.036*), dan juga pada kelompok preeklampsia dengan kelompok perlakuan (p=0.036*). Sedangkan rerata body neuron K(-) 72.86±28.46, pada K(+) 35.02±11.66, dan pada (P) 72.56±25.12. Didapatkan perbedaan signifikan pada kelompok normal dengan kelompok preeklampsia (p=0.001*), dan juga pada kelompok preeklampsia dengan kelompok perlakuan (p=0.001*). Kesimpulan: L-Arginine berpengaruh terhadap sel astrosit dan body neuron pada mencit (Mus musculus) model preeklampsia yaitu meningkatnya jumlah sel astrosit dan body neuron. Kata Kunci: L-Arginin, Sel Astrosit, Body Neuron, Preeklampsia Effect of L-Arginine on Astrocytes and Body Neurons in Mice Brain (Mus Musculus) Preeklampsia Model Robert Ridwan, Sri Sulistyowati, Supriyadi Hari R Department of Obstetrics Gynecology Faculty of Medicine Sebelas Maret University Dr. Moewardi Hospital Surakarta ABSTRACT Background: Preeclampsia occupies the highest position in the group of hypertensive disorders in pregnancy in terms of negative effects on maternal and infant health. The imbalance between oxidant and antioxidant that causes oxidative stress and endothelial dysfunction play a role in the pathogenesis of preeclampsia. Increased acute blood pressure results in impaired cerebrovascular autoregulation, cerebral blood barrier disorders, and destructive protein and serum constituents in the brain. Impaired function of astrocytes as blood brain barrier and maintenance of extracellular ion balance, as well as apoptosis body neuron is a manifestation of cerebrovascular autoregulation disorder. L-Arginine has the role of the L-arginine-nitric oxide pathway in preeclampsia plays an important role in systemic hemodynamics and free radical regulation. This study aims to prove the effect of L-Arginine on astrocytes and body neurons in mice (Mus musculus) model of preeclampsia. Methods: This experimental analytical study was conducted on 30 Swiss females mice (Mus musculus) sixteen-day pregnant, healthy, 20-25 gram, to determine the difference between histopathology of astrocytic cells and body neurons in normal pregnant mice (K(-)), Preeclampsia (K(+)) and preeclampsia (P) pregnant mice who received L-Arginine therapy. Sampling preparations were performed on the mice brain which had previously been dissected and paraffin blocks were then given Hematoxylin Eosin (HE) staining. The results are assessed semiquantitatively by measuring the number of cells in each of the five fields of view. Data analysis using Oneway Anova and Post-Hoc test with Tukey test model with SPSS (Software Package for social Science) 21. Result: Average of astrocytes cells K(-) 36.53±15.72, at K(+) 24.84±8.70, and at (P) 30.46±9.23. There were significant differences in the normal group with preeclampsia group (p = 0.036*), and also in the pre-eclampsia group with treatment group (p = 0.036*). While the mean body neuron K(-) 72.86±28.46, at K(+) 35.02±11.66, and at (P) 72.56±25.12. There were significant differences in the normal group with preeclampsia group (p = 0.001*), and also in the preeclampsia group with treatment group (p = 0.001*). Conclusions: L-Arginine affects astrocytic cells and body neurons in mice (Mus musculus) model of preeclampsia that is the increasing number of astrocytes cells and body neurons. Keywords: L-Arginine, Astrocytes Cells, Body Neurons, Preeclampsia.

    Item Type: Thesis (Masters)
    Subjects: R Medicine > RG Gynecology and obstetrics
    Divisions: Fakultas Kedokteran > Spesialis Obstetri Ginekologi
    Depositing User: Arief Atmojo
    Date Deposited: 21 Dec 2017 12:16
    Last Modified: 21 Dec 2017 12:16
    URI: https://eprints.uns.ac.id/id/eprint/37772

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