Potensi Isolat Fungi Trichoderma Lignolitik dalam Biodegradasi Plastik LDPE (Low Density Polyethylene)

Hikmah, Mamluatul (2017) Potensi Isolat Fungi Trichoderma Lignolitik dalam Biodegradasi Plastik LDPE (Low Density Polyethylene). Other thesis, Universitas Sebelas Maret.

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    Abstract

    Plastic is a synthetic polymer that is difficult to degrade because the plastic has a high molecular mass density. O ne of the most widely used type of plast ic is low density polyethylene or LDPE (low density polyethylene). Biodegradation process can be used as an alternative to degradation because the process is environmentally friendly. Some fungi of the genus Trichoderma are known to have a role in plastic biodegradation. This study aimed to find out how the potential of Trichoderma spp. lignolytic isolates in LDPE plastic biodegradation . Five Trichoderma isolates were screened by growing on MSMB (mineral salt medium broth) emulsified LDPE powder , with 35 days incubation at 30° C and shaking at 80 rpm. The screening results show ed that TL1, TL4, and TL5 we re the three most potential isolates, indicated by the growth marked by increasing colony size on screening media with LDPE as the only carbon source. Thre e selected isolates were tested for their biodegradability by growing the isolates in MSMA medium (medium agar salt agar), then 4 sheets of sterile 1x3 cm 2 LDPE placed on the surface of the colony in petri with incubation for 5, 15, 25 and 35 days. At each inc ubation period, the LDPE sheet wa s harvested, sterilized, and dried to be weighed in constant dry weight . The degredability assessment wa s done by measuring the weight loss of LDPE sheets after biodegradation treatment. In this research, the degradabil ity percentage values of TL1, TL4, TL5 and control ar e 4.87%, 7,12%, 7,51% and 0,28% respectively. Assessme nt of biodegradation potential wa s also done by observing the surface morphology of LDPE film of each treatment of isolate and control before and after biodegradation by SEM technique (scanning electron microscopy). Visual micrograph results show ed the appearance of damage and unevenness on the surface of the post - degradation film. Key words: Biodegradation, Plastic, Po l yethylene , LDPE, Fungi, Tric hoderma.

    Item Type: Thesis (Other)
    Subjects: Q Science > Q Science (General)
    Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam > Biologi
    Depositing User: Arief Atmojo
    Date Deposited: 11 Dec 2017 10:51
    Last Modified: 11 Dec 2017 10:51
    URI: https://eprints.uns.ac.id/id/eprint/36989

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