APLIKASI PENGGUNAAN KITOSAN PADA AKLIMATISASI PLANTLET ANGGREK BULAN [Phalaenopsis amabilis (L.) Blume.]

Aeni, Laela (2017) APLIKASI PENGGUNAAN KITOSAN PADA AKLIMATISASI PLANTLET ANGGREK BULAN [Phalaenopsis amabilis (L.) Blume.]. Other thesis, Universitas Sebelas Maret.

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    Abstract

    Moth orchid [Phalaenopsis amabilis (L.) Blume.] is a widely cultivated orchid species as a breeding parent. In vitro culture is the most effective propagation procedure for mass production of orchid. Acclimatization is an important step in transition process of plantlets from in vitro to ex vitro condition. On in vitro condition, morphology and physiology of plantlets were a susceptible characteristic, such as thin cuticle, stomata function was not optimal and high transpiration rate. Chitosan is used as substances that influence the orchid moth plantlets acclimatization. This research was conducted to determine the effect of spraying chitosan on the success moth orchid plantlet acclimatization and determine the optimum concentration of chitosan that can help the success of moth orchid plantlets acclimatization. This research used a completely randomized design (CRD) with various concentrations of chitosan (0 g/L; 0,5 g/L; 0,75 g/L; 1 g/L). Observation made during the 56 DAP (Days After making Planting). The parameters observed were amount of bud and leaf colour was analyzed according to describe, and survival rate, length and width of leaf, number of leaf, density and index of stomata were analyzed with Analysis of Varience (ANOVA), if there was a significant different were between treatment group then followed with Duncan Multiple Range Test (DMRT) at level significant 5 %. The result showed that chitosan influences significant on increased density of stomata and reduced length of leaf. Chitosan didn’t influence on width of leaf, number of leaf, amount of bud, leaf colour and index of stomata. The concentration of chitosan at 0,5 g/L showed a positive influences on the successfully of moth orhid plantlet acclimatization. Keyword: Acclimatization, Moth Orchid (Phalaenopsis amabilis (L.) Blume.), Chitosan

    Item Type: Thesis (Other)
    Subjects: Q Science > QK Botany
    Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam > Biologi
    Depositing User: Zahrotun Hanifah
    Date Deposited: 27 Oct 2017 19:57
    Last Modified: 27 Oct 2017 19:57
    URI: https://eprints.uns.ac.id/id/eprint/35690

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