HUBUNGAN KERAGAMAN POLA PITA RNA TOTAL DENGAN VIRULENSI FUSARIUM ASAL SOLANACEAE

ULYA, SITI IFADATUL (2017) HUBUNGAN KERAGAMAN POLA PITA RNA TOTAL DENGAN VIRULENSI FUSARIUM ASAL SOLANACEAE. Other thesis, Universitas Sebelas Maret.

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    Abstract

    The existence of double-stranded RNA mycovirus inducing hypovirulence of fungus was reported on some pathogenic fungi and potential to develope as a biological control agent. The purpose of this study was to determine the diversity of Fusarium from Solanaceae based on the total RNA pattern related the virulence of Fusarium. Total of 32 isolates of Fusarium were isolated from Solanaceae field and identified morphologically and virulence tested on previous study. Each isolats was subcultured on 20ml Potato Dextrose Broth (PDB) and filtered after ±14 days. total RNA was extracted from Fusarium mycelium to total RNA pellets, electrophoresed on 0,7% agarose gel and visualized using UV transiluminator. Total of seven isolates of Fusarium showed the presence of RNA. A1, A4, A5, A8, and A10 total RNA pattern showed the band size from 6.6 to 9.4 bp. A10, B3 and B9 total RNA pattern showed the band size from 4.4 to 6.6 kbp. Based on the results, only B3 was hypovirulence. A1, A4, A8 and B9 had medium virulence and A5 and A10 had a high virulence. This condition may be caused due to infection of mycovirus in some isolates was symptomless.

    Item Type: Thesis (Other)
    Subjects: S Agriculture > S Agriculture (General)
    Divisions: Fakultas Pertanian
    Fakultas Pertanian > Agroteknologi
    Depositing User: Fransiska Meilani f
    Date Deposited: 10 May 2017 14:45
    Last Modified: 10 May 2017 14:45
    URI: https://eprints.uns.ac.id/id/eprint/33828

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