PENGARUH PEMBERIAN VASCULAR ENDOTHELIAL GROWTH FACTOR 121 REKOMBINAN TERHADAP EKSPRESI SEL NATURAL KILLER DESIDUA PADA MENCIT (MUS MUSCULUS) MODEL PREEKLAMPSIA

Indra H, Alexander (2017) PENGARUH PEMBERIAN VASCULAR ENDOTHELIAL GROWTH FACTOR 121 REKOMBINAN TERHADAP EKSPRESI SEL NATURAL KILLER DESIDUA PADA MENCIT (MUS MUSCULUS) MODEL PREEKLAMPSIA. Masters thesis, Universitas Sebelas Maret.

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    Abstract

    ABSTRACT Preeclampsia is a disease in pregnancy that still one of the main causes of maternal and infant mortality. The incidence is 3% worldwide. One of preeclampsia theory is implantation failure caused by maternal maladaptation immun system decidual Natural Killer cells (dNK cells). dNK cells also known as a producers of proangiogenik Vascular Endothelial Growth Factor VEGF in early implantation period. Several studies shown the incerase of dNK cells and lower VEGF expression in the preeclamptic women. Administration of recombinant VEGF121 expected to improve the levels of VEGF and so dNK cells. This study is an experimental analytic study with randomized control trial (RCT) design, aimed to determine the effect of external VEGF, recombinant VEGF121, to the improvement of dNK cells expressions in preeclampsia. The samples collect from 30 pregnant mice (n=10) which divided into three groups: normal pregnant mice group, group of preeclamptic pregnant mice model without recombinant VEGF121 administration and group of preeclamptic pregnant mice models with recombinant VEGF121 administration. The recombinant VEGF121 administration was perform on the -12 to -15 of mice gestation day by intravenous injection 125 mg/kg/day, continued with sampling on day -16. Examination of dNK cells using immunohistochemical staining anti CD56 antibody for CD56. Data analysis using Kruskal Wallis Test and Mann Whitney Test with SPSS (Software Package for Social Science). From examination we found significant decrease of CD56 in the group of preeclamptic pregnant mice models with administration of recombinant VEGF121 compared to the group of preeclamptic pregnant mice model without recombinant VEGF121 administration (p = 0.023; <0.05). So the conclusion is the administration of recombinant VEGF121 will decreased decidual NK cells expression in preeclamptic mice model. Keywords: Preeclampsia, decidual NK cells, recombinant VEGF121, mice.

    Item Type: Thesis (Masters)
    Subjects: R Medicine > RG Gynecology and obstetrics
    Divisions: Fakultas Kedokteran
    Depositing User: Figur Hisnu Aslam
    Date Deposited: 05 Apr 2017 14:14
    Last Modified: 05 Apr 2017 14:14
    URI: https://eprints.uns.ac.id/id/eprint/33251

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