APLIKASI ENZIM POLIGALAKTURONASE DAN SELULASE PADA KLARIFIKASI SARI BUAH NAGA SUPER MERAH (Hylocereus costaricensis)

RISKI, HIDAYAH PUJI (2016) APLIKASI ENZIM POLIGALAKTURONASE DAN SELULASE PADA KLARIFIKASI SARI BUAH NAGA SUPER MERAH (Hylocereus costaricensis). Other thesis, Universitas Sebelas Maret.

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    Abstract

    The purpose of this study is to determine the effect and interaction of polygalacturonase enzyme bacterial isolate pectinolitic AR2 at concentrations of 0%; 0.06%; 0.09% and 0.1% and cellulase enzyme bacterial isolate cellulolitic S6 at concentrations of 0%; 0.1% and 0.13% in the clarification of super red dragon fruit juice with parameters of pH, total dissolved solids, viscosity, and transmittance. Polygalacturonase enzyme hydrolyze pectin into galacturonat acid and another galacturonan and cellulose enzyme hydrolyze cellulose into sub-units glucose. The results of this research obtained enzyme activity of polygalacturonase and cellulase was 0.161 U/ml and 0.027 U/ml respectively by partial purification. Addition of polygalacturonase and cellulase enzyme can increase pH, total dissolved solids, and transmittance, but decrease the viscosity value of super red dragon fruit juice. Sample with addition of polygalacturonase enzyme 0.06% and cellulase enzyme 0.13% with value of total dissolved solids 12.267 oBrix, viscosity 38.75 cP, transmittance 1.21 (% T535), and the pH value is 6.083 as selected samples. The highest transmittance value of the sample with the addition of polygalacturonase and cellulase enzyme at concentration 0.06% and 0.13% with a value of 1.21 (% T535)

    Item Type: Thesis (Other)
    Subjects: Q Science > QK Botany
    Divisions: Fakultas Pertanian > Ilmu dan Teknologi Pangan
    Depositing User: Retno Andriani
    Date Deposited: 23 Nov 2016 08:57
    Last Modified: 23 Nov 2016 08:57
    URI: https://eprints.uns.ac.id/id/eprint/29524

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