Pengaruh Pemberian Ekstrak Kulit Manggis (Garcinia mangostana L.) dalam Mencegah Kerusakan Histopatologi Ginjal Tikus Putih (Rattus norvegicus) yang Diinduksi Gentamisin

Pratama, Rio Nanda (2016) Pengaruh Pemberian Ekstrak Kulit Manggis (Garcinia mangostana L.) dalam Mencegah Kerusakan Histopatologi Ginjal Tikus Putih (Rattus norvegicus) yang Diinduksi Gentamisin. Other thesis, Universitas Sebelas Maret.

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    Abstract

    ABSTRACT Background: The use of medicinal plants has been increasing in recent years following development of herbal industry and evidence on adverse effects of several chemical drugs, including nephrotoxic effect. Research on herbs with nephroprotective effect are needed to offer alternative therapy for drug-induced renal diseases. Mangosteen pericarp extract contains xanthone which has antiinflammation effect to prevent renal damages. This research aimed to examine the nephroprotective effect of mangosteen pericarp extract on renal structure. Methods: This research was a laboratory experiment with posttest only controlled group design. Subjects were white male Wistar-strain rats (Rattus norvegicus) aged 2-3 months and weighed 200-230 grams. Subject were randomly divided into five treatment groups. Normal control group was given 0.5% NaCMC, while negative control group was given nothing. Treatment groups 1, 2 and 3 were given mangosteen pericarp extract at doses of 62.5 mg/kg-BW, 125 mg/kg-BW, and 250 mg/kg-BW, respectively. Treatments were maintained for 14 days, however negative control group and treatment groups 1, 2, and 3 were induced halfway by gentamicin 100 mg/kg-BW. Subjects were later terminated and their kidneys were preparated by hematoxicylin eosin staining. Histopathological renal damages were semi-quantitatively measured by identification of pyknosis, karyorrhexic and karyolysis areas in proximal tubulus cells and were compared using one-way ANOVA test and followed by LSD test. Result: Result from one-way ANOVA test showed that proximal tubulus cellular damage differed significantly across five groups (p < 0.001). LSD tests further showed a significant difference between negative control group and other groups (p < 0.001). Renal damage in normal control group was also found to differ significantly from those of treatment group 1 and 3 (p < 0.001). No significant difference was found between normal control and treatment group 2 (p = 0.972). Conclusion: Mangosten pericarp extract (Garcinia mangostana L.) could prevent histopathological renal damage in gentamicin-induced white rats (Rattus norvegicus). Keywords: Mangosteen pericarp extract, Renal histopathology, Gentamicin.

    Item Type: Thesis (Other)
    Subjects: Q Science > Q Science (General)
    Divisions: Fakultas Kedokteran
    Depositing User: Nanda Rahma Ananta
    Date Deposited: 14 Nov 2016 14:24
    Last Modified: 14 Nov 2016 14:54
    URI: https://eprints.uns.ac.id/id/eprint/28614

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