Penapisan dan Karakterisasi Awal Senyawa Bioaktif Lectin Alga Merah Pesisir Pantai Gunung Kidul, Yogyakarta

NURHAYATI, TRI (2016) Penapisan dan Karakterisasi Awal Senyawa Bioaktif Lectin Alga Merah Pesisir Pantai Gunung Kidul, Yogyakarta. Other thesis, Universitas Sebelas Maret.

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    Abstract

    SCREENING AND CHARACTERIZATION OF LECTINS RED ALGAE FROM GUNUNGKIDUL COAST OG YOGYAKARTA Tri Nurhayati H0911063 Abstract Exstracts from 13 species red marine algae collected along Gunungkidul coast of Yogyakarta by precipitation ammonium sulphate. This crude exstract lectin were examined for hemaglutination activity using native and enzyme-treated rabbit and human (grub A,B and O) erithrosit. Strongest activity was detected in exstract from Anhfettiopsis linearis then Liagora farinosa. The hamglutination of six Rhodophyta species were examined for sugar-binding specifity, pH and Temperature-stability, and divalent cation-independency of hemaglutination. In a hemaglutination-inhibition test with various monosaccharides, oligosaccharides and glycoprotein, none of the hemaglutinins had affinity for monosaccharides and oligosaccharides. On the other hand, all of the hemagglutinins activities were inhibited by some glycoprotein. The inhibition profiles with glycoproteins were different depending on hemagglutinin species. The activities of these algal hemaglutinins were stable over a wide range of pH but not stable over wide range temperature except Palmaria palmata and independent of the presence of divalent cations. These result indicate that Gunungkidul marine algae are good source of novel and useful lectin. Keywords : Lectins, Red Algae, Stability, Carbohidrat-binding specificity.

    Item Type: Thesis (Other)
    Subjects: S Agriculture > SB Plant culture
    Divisions: Fakultas Pertanian
    Fakultas Pertanian > Ilmu dan Teknologi Pangan
    Depositing User: fransiska siska meilani
    Date Deposited: 27 Jan 2016 14:43
    Last Modified: 27 Jan 2016 14:43
    URI: https://eprints.uns.ac.id/id/eprint/23267

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