Efek Pemberian Ekstrak Etanol Daun Kelor (Moringa oleifera Lamk.) dalam Mencegah Penurunan Memori Spasial Tikus (Rattus norvegicus) yang Diinduksi Stres. Skripsi. Fakultas Kedokteran, Universitas Sebelas Maret, Surakarta.

SALAMAH, NOVA SARI NUR (2015) Efek Pemberian Ekstrak Etanol Daun Kelor (Moringa oleifera Lamk.) dalam Mencegah Penurunan Memori Spasial Tikus (Rattus norvegicus) yang Diinduksi Stres. Skripsi. Fakultas Kedokteran, Universitas Sebelas Maret, Surakarta. Other thesis, UNIVERSITAS SEBELAS MARET.

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    Abstract

    ABSTRAK Nova Sari Nur Salamah, G.0010139, 2014. Efek Pemberian Ekstrak Etanol Daun Kelor (Moringa oleifera Lamk.) dalam Mencegah Penurunan Memori Spasial Tikus (Rattus norvegicus) yang Diinduksi Stres. Skripsi. Fakultas Kedokteran, Universitas Sebelas Maret, Surakarta. Latar Belakang: Daun kelor (Moringa oleifera Lamk.) memiliki kandungan antioksidan yang dapat melindungi fungsi memori dari paparan stres, yaitu flavonoid, tanin, dan vitamin. Penelitian ini bertujuan membuktikan bahwa pemberian ekstrak etanol daun kelor dapat mencegah penurunan memori spasial tikus (Rattus norvegicus) yang diinduksi stres. Stres ditimbulkan oleh bising intermiten. Metode Penelitian: Penelitian ini bersifat eksperimental murni dengan post test only controlled group design. Penelitian dilakukan di laboratorium Sentral MIPA Biologi Universitas Sebelas Maret, Surakarta. Sampel berupa 30 tikus jantan galur Wistar berumur 8 minggu. Sampel dibagi dalam 5 kelompok. Kelompok kontrol (KK) diberi akuades, kelompok stres (KS) diberi stres bising intermiten. Tiga kelompok perlakuan diberi ekstrak etanol daun kelor dilanjutkan stres bising intermiten. Kelompok perlakuan 1 (KP1) diberi ekstrak dosis 250 mg/kgBB/hari, kelompok perlakuan 2 (KP2) dosis 500 mg/kgBB/hari, kelompok perlakuan 3 (KP3) dosis 750 mg/kgBB/hari. Bising intermiten diberikan selama 4 jam/hari dengan intensitas 100 dB. Perlakuan dilakukan selama 21 hari. Pada hari ke-22 sampai hari ke-26, memori spasial tikus dinilai berdasarkan escape latency dalam Morris water maze. Tiap tikus menjalani 4 trial perhari. Data dianalisis menggunakan uji two-way repeated ANOVA (α = 0,05) dilanjutkan uji post hoc LSD (α = 0,05). Hasil Penelitian: Rerata escape latency tikus antarkelompok berbeda secara bermakna (p < 0,05). Hasil uji post hoc LSD pada KS-KP1, KS-KP2, KS-KP3 tidak berbeda bermakna (p > 0,05). Escape latency KP1, KP2, dan KP3 lebih rendah daripada KS pada hari pengamatan ke-1, 3, dan 5, selain itu KP2 memiliki escape latency paling rendah pada hari ke-4 dan 5. Rerata escape latency tikus antarhari pengamatan berbeda bermakna (p < 0,001). Tidak ada interaksi antara variabel kelompok perlakuan dan hari pengamatan. Simpulan Penelitian: Pemberian ekstrak etanol daun kelor (Moringa oleifera Lamk.) dapat mencegah penurunan memori spasial tikus yang diinduksi stres. Kata kunci: memori spasial, daun kelor, Morris water maze, bising intermiten v ABSTRACT Nova Sari Nur Salamah, G.0010139, 2014. Effect of Oral Administration Leaves of Moringa oleifera Lamk. Ethanolic Extract in Preventing Spatial Memory Impairment in Stres-induced Rat. Mini Thesis. Faculty of Medicine, Sebelas Maret University, Surakarta. Background: Leaves of Moringa oleifera Lamk. contain various antioxidant substances such as flavonoids, tannins, and vitamins, which they were thought to have protective effect of learning and memory impairment induced by stress. The present study aimed to evaluate the influence of leaves of Moringa oleifera Lamk. ethanolic extract in preventing spatial memory impairment in stres-induced rat. Methods: The present study was an experimental research with post test only controlled group design. Samples were 30 male rats, Wistar strain, 8 weeks old age. Samples were divided into 5 groups. In this study, stress were generated by a 100 dB daily-4 hours intermittent noise. Control group were given aquadest, stress group were subjected to intermittent noise. Treatment groups were subjected to intermittent noise and given leaves of Moringa oleifera Lamk. ethanolic extract. Treatment 1 group were given extract with the dose of 250 mg/kg body weight, treatment 2 group with the dose of 500 mg/kg body weight, and treatment 3 group with the dose of 750 mg/kg body weight. Treatment were held in 21 days. Spatial memory of rats were evaluated using Morris water maze test in five consecutive days. Escape latency were analyzed using the two-way repeated ANOVA (α = 0,05) followed by post hoc LSD test (α = 0,05). Results: Escape latency among groups were statistically different (p < 0,05). There were no statistically significant difference between stress - treatment groups (p > 0,05). Escape latency of treatment groups were lower than stress group in 1st, 3rd, and 5th test day and treatment 2 group had lowest escape latency among groups in 4th and 5th test day. Escape latency among observation days were statistically different (p < 0,001). There were no interaction between tratment group with test day. Conclusion: Oral administration leaves of Moringa oleifera Lamk. ethanolic extract prevent spatial memory impairment in stres-induced rat. Keywords: spatial memory, leaves of Moringa oleifera Lamk., Morris water maze, intermittent noise

    Item Type: Thesis (Other)
    Subjects: R Medicine > R Medicine (General)
    Divisions: Fakultas Kedokteran
    Fakultas Kedokteran > Pendidikan Kedokteran
    Depositing User: Afifah Shobrina
    Date Deposited: 03 Oct 2015 19:34
    Last Modified: 03 Oct 2015 19:34
    URI: https://eprints.uns.ac.id/id/eprint/20358

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