PENAMBAHAN ASAM ASKORBAT SEBAGAI INDUKSI DIFERENSIASI KONDROGENIK SEL PUNCA MESENKIMAL YANG BERASAL DARI TALI PUSAT, SEBUAH STUDI IN VITRO PADA TIKUS

SAPUTRA, RHYAN DARMA (2013) PENAMBAHAN ASAM ASKORBAT SEBAGAI INDUKSI DIFERENSIASI KONDROGENIK SEL PUNCA MESENKIMAL YANG BERASAL DARI TALI PUSAT, SEBUAH STUDI IN VITRO PADA TIKUS. Other thesis, Universitas Sebelas Maret.

[img]
Preview
PDF
Download (111Kb) | Preview

    Abstract

    Abstract Background: Adult articular cartilage has a limited ability to spontaneously heal when damaged by trauma or disease. A variety of reasons for this lack of reparative response have been postulated, including the absence of a fibrin clot, the inability of chondrocytes to migrate into the site of injury, and the avascular nature of cartilage. Despite the inferior ability for chondrocyte to regenerate, there is a chance in tissue engineering to manipulate chondrocyte regeneration. Many studies has been develop a great interest in management of articular cartilage regeneration. Nowadays, the most promising methode is by developing mesenchymal stem cells and progenitor cells. Mesenchymal Stem Cells (MSCs) are the most common source for tissue repair, it can be isolated from bone marrow, adipose tissue, umbilical cord, synovium, periosteum, and muscle tissue. Umbilical cord is an important source of stem cells that are easy to obtain, cheap, and do not have any problems either in a medical or ethical nature. Umbilical cord mesenchymal stem cells (UCMSCs) can be obtain from Wharton’s Jelly, which is the matrix from umbilical cord tissue itself. Several studies shown that MSCs had a highly regulating process involving cell proliferation, migration, differentiation, and maturation leads to the production and sustenance of most cell lineage in adult organisms. This process sometimes uncontrollable and not easy to predict. Some in vitro studies from bone marrow derived MSCs, has shown that the augmentation of TGF – β and ascorbic acid can help to control and stimulate phenotype differentiation from MSCs to chondrocyte. Based on these report, we try to develop a low cost source methode of chondrocyte phenotype differentiation by using the umbilical cord derived mesenchymal stem cells (UCMSCs) and augmentation with ascorbic acid only. Objective : Determine the chondrocyte differentiation ability from umbilical cord derived mesenchymal stem cells in the wharton’s jelly in term determine the mechanism of trans differentiation of UCMSCs in wharton’s jelly to the chondrocyte cells. The aim of our study is also define the use of ascorbic acid to induced chondrogenic differentiation of UCMSCs into the unipotent chondrocyte cells. Material and Methods: This study conduct is an in vitro experimental study, using a pure strain wistar rat models. The umbilical cord derived mesenchymal stem cells obtained in wharton’s jelly isolated from 19 days pregnancy of rat models. This wharton’s jelly is done for pirmary cell culture, subculture, then we try to control the ability of differentiation of this adult mesenchymal stem cells. All the cell culture using a Dulbecco’s modified Eagle’s Medium + fetal bovine serum + antibiotics and fungizone. Control for UCMSCs differentiation is performed by augmentation of ascorbic acids into the culture media in terms of dose dependent, with an addition of ascorbic acids 50 µg /ml complete media or 100 µg / ml complete media. each subject then performed for pathological examination using alcyan blue staining and immunocytochemistry examination for CD44 phenotype marker and collagen type IV marker. CD44 is an identification markers for mesenchymal stem cells. Identification for chondrogenic differentiation is examine by detection of collagen type IV in cell culture. Results and Discussion: This study shown that wharton’s jelly from the umbilical cord can beused as a source of mesenchymal stem cells. The umbilical cord derived mesenchymal stem cells shows a good expression in CD44 phenotype marker. The cell culture itself perfomed in 14 day to achieve stable and confluence stem cells. After achieved 2nd passage of the cell culture, then we performed an ascorbic acid augmentation to control the cell differentiation. The ascorbic acid augmentation shows a better result to controll chondrogenic differentiation. The collagen type IV immuncytochemistry examination shows a good expression of collagen type IV in cell culture. Conclusions: The use of umbilical cord derived mesenchymal stem cells is considerable in tissue engineering. This source of stem cells is easy to obtain, cheap, and do not have problem in either medical nature for its use in cell regeneration. The umbilical cord stem cells itself can be controlled for the chondrogenic differentiation by the terms using an ascorbic acid. Further study need to perform to establish its use in cartilage repair. Keywords : Umbilical Cord Derived Mesenchymal Stem Cells, Chondrogenic Differentiation, Ascorbic Acid

    Item Type: Thesis (Other)
    Subjects: R Medicine > R Medicine (General)
    Divisions: Fakultas Kedokteran > Pendidikan Kedokteran
    Depositing User: Minardi Aris
    Date Deposited: 27 Sep 2015 14:31
    Last Modified: 27 Sep 2015 14:31
    URI: https://eprints.uns.ac.id/id/eprint/19612

    Actions (login required)

    View Item