Probiotic Effects of Mortality, Degree of Intestinal Inflammation, and IgA Levels in Sepsis Mice Model

Indrayanto, Yoseph and Prasetyo, Diding Heri (2013) Probiotic Effects of Mortality, Degree of Intestinal Inflammation, and IgA Levels in Sepsis Mice Model. Majalah Kedokteran Bandung, 45 (1).

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    Abstract

    Sepsis results in changes to the microbiology of the gastrointestinal tract, leading to a loss of commensal flora and an overgrowth of potentially pathogenic bacteria. Administering probiotics to sepsis patients may restore balance T to the microbiota and have positive effects on immune function and gastrointestinal structure and function. This study was aimed to evaluate the effect of probiotic on mortality, intestinal inflammation and immunoglobulin A (IgA) serum level in mice model of sepsis. This study was an experimental research laboratory, with 36 male Balb/C mice were divided into: control, sepsis, and sepsis+probiotic groups. The study was conducted at Histology and Biomedical Laboratory, School of Medicine, Sebelas Maret University, Surakarta, in January to April 2012. Sepsis was induced in the male Balb/C mice using an intraperitoneally (i.p) injection of cecal inoculum (200 mg/’ l<gBW), for sepsis mice model and sepsis with probiotic. Control mice were not inoculation during the study. Detection of intestinal inflammation with hematoxylin and eosin (HE) staining on 8th day. Mortality perceived until 8th day. IgA serum level measurement with enzyme-linked immunosorbent assay. Two-tailed Fisher exact test for the analysis of mortality, Kruskal-Wallis test for the intestinal inflammation, one way analysis of variance for IgA serum level. Probiotic showed no significantly mortality (10% vs 40%, p=0.065) compared to sepsis group. Probiotic showed significantly decreased intestinal inflammation compared to sepsis group (p<0.ool). Control mice showed levels of IgA 35.82i4.55 ng/mL. Probiotic showed significantly increased lgA levels (65.07i34.97 ngt'mL vs 6.2ofi:5.8O ng/mL, p<0.ooi) compared to sepsis group. In conclusions, probiotic decrease intestinal inflammation and mortality, on the other hand increase IgA level in mice model of sepsis. [MKB. 201 3;45('l ): MP5] Key words: lgA_. inflammation, probiotic, sepsis

    Item Type: Article
    Subjects: R Medicine > R Medicine (General)
    Divisions: Fakultas Kedokteran
    Depositing User: Arissa Aprilia Nurcahyani
    Date Deposited: 10 May 2014 18:25
    Last Modified: 10 May 2014 18:25
    URI: https://eprints.uns.ac.id/id/eprint/15927

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