The Expression of CD8+ and MHC-I in Cervical Cancer with HPV Infection

Prayitno, Adi and Asnar, Elyana and Astirin, Okid Parama and Putra, Suhartono Taat (2013) The Expression of CD8+ and MHC-I in Cervical Cancer with HPV Infection. Journal of Cancer Therapy, 4. pp. 15-18.

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    Introduction: The immunity against cervical cancers is many unknown. Major histocompatability complex class I (MHC-I) with the alpha domain has binding site for Cytotoxic T cells (CD8+) to target cells. The objective of this ex- periment is to known the expression of CD8+ and MHC-I in cervical cancer with HPV infection. Method: DNA was isolated from seventeen sample cervical cancer tissues frozen section. Diagnose related with HPV was made by PCR. Paraffin block of the tissues was cut in thoroughly cleaned cryotome and place in glass plate that covered with poly-elysine. The immunohistochemistry is done with monoclonal antibody again MHC-I and CD8+ with TSA-indirect method. The power of expression was counted the number of positive cells to express the MHC-I and CD8+ every 100 cells per one view look. Result: The Result of this experiment show that expression of MHC-I and CD8+ in cervical cancer with HPV infection is in mild category (30% - 70%). Conclusion: Immunity against uterine cancer that played by MHC-I and CD8+ are in milt category. The experiment that related with uterine cancer immunology is suggested.

    Item Type: Article
    Subjects: Q Science > Q Science (General)
    R Medicine > R Medicine (General)
    Divisions: Lembaga Penelitian dan Pengabdian Kepada Masyarakat - LPPM
    Depositing User: Anis Fagustina
    Date Deposited: 08 May 2014 10:08
    Last Modified: 08 May 2014 10:08

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