Plasma-activated immobilization of biomolecules onto graphite- encapsulated magnetic nanoparticles

Saraswati, Teguh Endah and Ogino, Akihisa and Nagatsu, Masaaki (2012) Plasma-activated immobilization of biomolecules onto graphite- encapsulated magnetic nanoparticles. elsevier Carbon, 50 (3). pp. 1253-1261.

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    Abstract

    We describe the amino group surface functionalisation of graphite-encapsulated iron compound nanoparticles by radio frequency (RF) plasma processing followed by oxidized dextran immobilization. We have found that surface treatment using plasma represents an important step before biomolecules immobilization. After plasma treatment, the dispersion property of nanoparticles in dextran solution in water was significantly improved. The successful dextran immobilization was confirmed by X-ray photoelectron spectroscopy (XPS) and high resolution-transmission electron microscopy (HR-TEM) analyses followed by amino group derivatization using 4-(trifluoromethyl)-benzaldehyde (TFBA). As an evidence for covalent bonding between nanoparticles and dextran, the area percentage of deconvoluted C N peak at ∼389.6 eV increased from 0% to 10.53 ± 1.30% with increasing the dextran concentration. The result is consistent with the evidenced decreasing of the free amino group percentage from 68.09 ± 5.10% to 14.73 ± 5.89% on the nanoparticle surface after dextran immobilization.

    Item Type: Article
    Subjects: Q Science > Q Science (General)
    Q Science > QD Chemistry
    T Technology > T Technology (General)
    T Technology > TP Chemical technology
    Divisions: Lembaga Penelitian dan Pengabdian Kepada Masyarakat - LPPM
    Depositing User: Anis Fagustina
    Date Deposited: 08 May 2014 04:57
    Last Modified: 08 May 2014 04:57
    URI: https://eprints.uns.ac.id/id/eprint/15344

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