(B. Pertanian) DNA Sequencing and Determining of Tanin and Seed Gens in Order To Find New Superior Varieties of Salak Toward DNA Recombinant

Nandariyah, and Hartati, Sri and Wartoyo, and Pardono , (2012) (B. Pertanian) DNA Sequencing and Determining of Tanin and Seed Gens in Order To Find New Superior Varieties of Salak Toward DNA Recombinant. .

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    Abstract

    Indonesia is a center of origin and cultivation of Salak (Salacca zalacca Gaertner Voss) with a wide range of quality fruit. Salak is one type of fruit that have a significant economic value as a commodity for domestic and export trade. Fruits are consumed as fresh fruit and can be made as processed food. Taste determining the selling fruits of salak. One of the taste determining is tannin content of fruit that causes fruit to taste sepet less preferred by consumers. The first year research aims to determine the diversity of salak accessions based on the fruit tannin content, Grouping accessions of salak based on tannin content and to find a specific marker of the molecular levels of tannins. Methods of laboratory research conducted at the Molecular Biology Laboratory of Food Technology Faculty of UGM with tannin analysis and molecular diversity analysis performed by the method of RAPD. Based on the content of tannin in the fruit, can be classified three types of accessions that contains high tannin, medium and low. By RAPD we grouping of accession salak in three groups there are Low, medium and high tannin content. Generally low tannin content is owned by a group from Yogyakarta pondoh salak, while the high tannin levels which is owned by the not pondoh salak except of Kediri and Bali salak. The results of molecular analysis showed the diversity of characters from the accession salak. Accession with a low tannin levels showed similarity type of DNA bands, including Gula pasir (Bali) salak, black Pondoh Sleman and Madura salak. Which yield high tannin (Kecandran salak) has a specific banding pattern different from the other accessions.

    Item Type: Article
    Subjects: S Agriculture > S Agriculture (General)
    Divisions: Lembaga Penelitian dan Pengabdian Kepada Masyarakat - LPPM
    Depositing User: Miranda Nur Q. A.
    Date Deposited: 06 May 2014 16:36
    Last Modified: 06 May 2014 16:36
    URI: https://eprints.uns.ac.id/id/eprint/15091

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