IDENTIFIKASIDAN UJIAKTIVITAS ANTIBAKTERI FRAKSI TERAKTIF DAUN PACAR KUKU (Lawsonia inermisLinn.) TERHADAP Staphylococcus aureus DAN Escherichia coli

Fitriana, Aprillia (2013) IDENTIFIKASIDAN UJIAKTIVITAS ANTIBAKTERI FRAKSI TERAKTIF DAUN PACAR KUKU (Lawsonia inermisLinn.) TERHADAP Staphylococcus aureus DAN Escherichia coli. Other thesis, UNIVERSITAS SEBELAS MARET.

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    Abstract

    Telah dilakukan uji aktivitas antibakteri fraksi teraktif daun pacar kuku (Lawsonia inermis Linn.) terhadap bakteri Staphylococcus aureus dan Escherichia coli. Fraksi teraktif diperoleh dari hasil pemisahan kromatografi vacum cair (KVC) ekstrak etanol daun pacar kuku (Lawsonia inermis Linn.) yang telah melalui tahap maserasi dengan pelarut etanol 70%. Proses KVC dilakukan dengan menggunakan tiga eluen yaitu n-heksan, etil asetat, dan etanol. Pengujian aktivitas antibakteri dilakukan dengan metode difusi agar. Komponen kimia dalam fraksi teraktif diidentifikasi melalui skrining fitokimia dengan metode kromatografi lapis tipis (KLT) dan kromatografi gas-spektrometer massa (GCMS). Uji aktivitas antibakteri menunjukkan bahwa ekstrak etanol dan fraksi-fraksi daun pacar kuku (Lawsonia inermis Linn.) mampu menghambat pertumbuhan bakteri Staphylococcus aureus dan Escherichia coli. Fraksi etil asetat adalah fraksi teraktif anti bakteri. Potensi anti bakteri fraksi etil asetat sebesar 3,58x10- 3% untuk Staphylococcus aureus dan 7,82x10-3% untuk Escherichia coli bila dibandingkan dengan antibiotik tetrasiklin HCl. Potensi antibakteri fraksi etil asetat daun pacar kuku lebih kecil daripada fraksi etil asetat kulit batang pacar kuku, namun lebih besar daripada fraksi etil asetat umbi lobak (Raphanus sativus) dan daun johar (Cassia siamea Lamk.) ditinjau dari nilai diameter daerah hambat. Identifikasi senyawa dalam fraksi teraktif melalui kromatografi lapis tipis (KLT) menunjukkan adanya golongan senyawa flavonoid, terpenoid, antrakuinon, saponin, dan asam lemak. Analisis GC-MS menunjukkan adanya senyawa dominan 2-pentanone,4-hydroxy-4 methyl, 2-propanol,1,1’-oxybis (CAS) dipropylene glycol, coumarin, dibutylphthalate, hexadecanoic acid, methyl ester, dan 10-octadecanoic acid. Kata kunci : Lawsonia inermis Linn., antibakteri, fraksi teraktif, identifikasi The test of antibacterial activity of the most active fraction of pacar kuku (Lawsonia inermis Linn.) leaf against Staphylococcus aureus and Escherichia coli has been conducted. The most active fraction was obtained by liquid vacuum chromatography crude extract of ethanol. The crude extract of ethanol was extracted through a maceration using ethanol 70%. Liquid vacuum chromatography was carried out using three eluents such as n-hexane, ethyl acetate, and ethanol. The test of antibacterial activity was evaluated by agar diffusion method. The most active fraction’s compounds were identified by screening phytochemical using thin layer chromatography and gass chromatography-mass spectrometry. The test of antibacterial activity indicated crude extract of ethanol and pacar kuku (Lawsonia inermis Linn.) leaf’s fractions have antibacterial activity against Staphylococcus aureus and Escherichia coli. Ethyl acetate fraction was the most active fraction for all bacterial tested. The potensial of anti bacterial of ethyl acetate fraction was 3.58x10-3% for Staphylococcus aureus and 7.82x10-3% for Escherichia coli, compared with tetracycline HCl. The potensial of antibacterial of ethyl acetate fraction pacar kuku leave was less than pacar kuku bark, but more than ethyl acetate fraction radish root (Raphanus sativus) and johar leave (Cassia siamea) from its zone of inhibition. Identification of the most active fraction’s compounds identified by thin layer chromatography showed ethyl acetate fraction contains flavonoids, terpenoids, anthraquinones, saponin, and fatty acid. The analysis of GC-MS showed ethyl acetate fraction contains 2-pentanone, 4-hydroxy-4-methyl, 2- propanol,1,1’-oxybis (CAS) dipropylene glycol, coumarin, dibutylphthalate, hexadecanoic acid, methyl ester, and 10-octadecanoic acid. Key word : Lawsonia inermis Linn., antibacterial, ethyl acetate fraction, identification

    Item Type: Thesis (Other)
    Subjects: Q Science > QC Physics
    Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam > Kimia
    Depositing User: Satria Nur Fauzi
    Date Deposited: 05 May 2014 22:47
    Last Modified: 05 May 2014 22:47
    URI: https://eprints.uns.ac.id/id/eprint/14984

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