Analysis of Rhizosphere Bacterial Community in Suppressive and Conducive Soils to Basal Rot of Garlic Based on PCR-RISA

Fatawi, Zainal D. and Hadiwiyono, and Widono , Salim (2012) Analysis of Rhizosphere Bacterial Community in Suppressive and Conducive Soils to Basal Rot of Garlic Based on PCR-RISA. BIOMIRROR BM An Open Access Journal, 3 (2). pp. 9-12. ISSN 0976 – 9080

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    Abstract

    Basal rot (Fusarium oxysporum f.sp. cepae) is an important disease of garlic in Tawangmangu Karanganyar Central Java Indonesia. Disease- suppressive could be found in some lands around disease-conducive lands in Tawangmangu. The role of rhizosphere bacterial cammunity to induce disease-suppressiveness has been studied extensively on many soil born pathogen. Among the many groups of such organisms are root-associated bacteria, which generally represent a subset of soil bacteria. Ribosomal Intergenic Spacer Analysis (RISA) is a Polymerase Chain Reaction (PCR)-based method as an useful assessment tool for the diversity analysis of microbes in the environment. Currently, the diversity analysis of rhizospere bacterial community of garlic planted on suppressive and conducive soil has been conducted. Conducive soil is the land where the disease incidence of basal rot of garlic at 1% or lower, whereas suppressive soil at 40% or higher. The diversity analysis was done through independent cultureable approach of PCR-RISA using universal primers of operon gene region of rRNA between small subunit (16S) and large subunit (23S) called intergenic spacer region (ISR). The diversity of rhizosphere bacteria from suppressive soil was different from that of conducive soil. The rhizosphere bacteria in suppressive soil was more diverse than those of conducive soil and the bacteria in healthy garlic rhizosphere was also more diverse than those in the diseased one.

    Item Type: Article
    Subjects: S Agriculture > S Agriculture (General)
    T Technology > T Technology (General)
    Divisions: Lembaga Penelitian dan Pengabdian Kepada Masyarakat - LPPM
    Depositing User: Anis Fagustina
    Date Deposited: 03 May 2014 20:51
    Last Modified: 03 May 2014 20:51
    URI: https://eprints.uns.ac.id/id/eprint/14806

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