PENGARUH PEMBERIAN EKSTRAK ETANOL DAUN TALOK (Muntingia calabura) SEBAGAI PENCEGAHAN INFEKSI BAKTERI Aeromonas hydrophila TERHADAP STRUKTUR HISTOLOGI INSANG IKAN NILA (Oreochromis niloticus)

Fatimah, (2013) PENGARUH PEMBERIAN EKSTRAK ETANOL DAUN TALOK (Muntingia calabura) SEBAGAI PENCEGAHAN INFEKSI BAKTERI Aeromonas hydrophila TERHADAP STRUKTUR HISTOLOGI INSANG IKAN NILA (Oreochromis niloticus). Other thesis, Universitas Sebelas Maret.

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    Abstract

    Abstract Motile Aeromonas Septicemia (MAS) is a disease caused by bacterial pathogens Aeromonas hydrophila. The conventional methods use antibiotics and chemicals to prevent the disease, although it has negative impact, like antibiotic resistance, antibiotic accumulation in the body, and environmental contamination. The usefulness of natural compounds is well known to prevent quorum sensing system, and it has not negative impact to the environment, and bacterial resistance. The aim of this research were to find out the optimum concentration of ethanol extract of jamaica cherry leaves (Muntingia calabura) that could be used to reduce the amount of bacteria in tilapia (Oreochromis niloticus) water culture, and to find out the effect of ethanol extract of jamaica cherry leaves (Muntingia calabura) to the survival rate, behavior, diet, and histological structure of the gills of tilapia (Oreochromis niloticus). Tilapia fish were immersed in the water involved by A. hydrophila bacterial with the concentration of colony bacteria, that was 1,5x108 colony/mL and talok leaf ethanol extract in different concentration, that were: 140 ppm, 150 ppm, 160 ppm, 170 ppm, dan 180 ppm. . The immersion treatment was done once in the first day of the research, it was immersed during 90 minute and continued with observation of the behavior of fish, like: swim pattern and eat pattern, for 3 weeks. The gill histology was observed by taking the gill of the fishes which could live after 3 weeks and the fishes which died before 3 weeks. Ethanol extract of jamaica cherry leaves (Muntingia calabura) could decreased the bacterial amount in the water was 170 ppm concentration. Ethanol extract of jamaica cherry leaves (Muntingia calabura) in 180 ppm concentration have the highest survival rate of the fishes. During immersion fish shown stress, it were appeared into the surface, moved randomly, and stayed in the bottom of the aquarium. The feeding response in fish were decrease after immersion, and increased in the sixth days after immersion. The gill histology of fish after expossured by jamaica cherry leaves (Muntingia calabura) ethanol extract, showed edema, hiperplasia, and lamella fusion. Keyword: Oreochromis niloticus, Motile Aeromonas Septicemia (MAS), quorum sensing, Aeromonas hydrophila, ethanol extract, jamaica cherry leaves (Muntingia calabura).

    Item Type: Thesis (Other)
    Subjects: Q Science > Q Science (General)
    Divisions: Lembaga Penelitian dan Pengabdian Kepada Masyarakat - LPPM
    Depositing User: Nurrahma Restia
    Date Deposited: 03 May 2014 18:12
    Last Modified: 03 May 2014 18:12
    URI: https://eprints.uns.ac.id/id/eprint/14535

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