ANALISIS KUANTITATIF NETROFIL SALIVA SEBAGAI BIOMARKER SEPSIS

Indrawijaya, Sony (2012) ANALISIS KUANTITATIF NETROFIL SALIVA SEBAGAI BIOMARKER SEPSIS. Masters thesis, Universitas Sebelas Maret.

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    Abstract

    Background: Sepsis can suffered in any person at all age groups, as well as healthy individuals and others who suffered from chronic disease. The incidence of sepsis in the developing and developed countries is quite high. Early and accurate diagnosis of sepsis is very important as early treatment is associated with better outcomes. Microbiological culture and new infection markers (procalcitonin and pro-adrenomedullin) are hampered by problems of time, cost and availability. Neutrophil saliva as a marker of infection disease or sepsis is more easily extracted, cost-effective and non-invasive. Objective: The aim of this study was to to describe the numbers and the phagositic activity of neutrophils saliva in sepsis patiens. Methode: This study was a cross sectional comparative study. After obtaining approval from the ethics committee of the Faculty of Medicine Sebelas Maret University, Dr. Moewardi General Hospital, Surakarta, we collected 90 samples, the healthy persons, SIRS and sepsis patients aged 19-59 years with a consecutive sampling. The samples were divided into three groups of 30 subjects each, each group was examined salivary neutrophils with flowcytometry methods and saliva swabs. Later in the analysis of the quantity and quality of the neutrophil. The data were tested with Anova /Kruskal-wallis test and Chi-square test. Result: There were different (p < 0,050) of the numbers netrofil saliva between SIRS gruop and normal group, and also between sepsis group and normal group, but there were not different betwen SIRS group and sepsis group (p > 0,050). Phagosytic activity from three groups were differ (p < 0,050). Conclusion: There were difference the numbers and the activity of neutrphil saliva between SIRS/sepsis group and normal/healthy group. Keywords: Sepsis, Neutrophil, Saliva, Flowcytometry. Latar Belakang: Sepsis dapat terjadi pada setiap orang di semua kelompok usia, individu sehat maupun yang menderita penyakit kronis. Angka kejadian sepsis di negara berkembang dan negara maju cukup tinggi. Diagnosis dini dan akurat pada sepsis sangat penting sebagai awal pengobatan dikaitkan dengan hasil yang lebih baik. Kultur mikrobiologis dan penanda infeksi yang baru (procalcitonin dan pro- adrenomedulin) terhambat oleh masalah waktu, biaya dan ketersediaan. Netrofil saliva sebagai penanda penyakit infeksi atau sepsis lebih mudah pengambilannya, hemat biaya, dan non-invasif. Tujuan: Penelitian ini bertujuan untuk mendeskripsikan jumlah dan aktivitas fagosit dari netrofil saliva pada pasien sepsis. Metode: Penelitian ini adalah comparative dengan rancangan cross sectional (potong lintang). Setelah mendapat persetujuan dari komite etik FK UNS RS Dr Moewardi Surakarta, dikumpulkan sebanyak 90 sampel penelitian, orang sehat/normal, pasien SIRS, dan pasien sepsis dengan usia 19–59 tahun secara consecutive sampling. Sampel dibagi menjadi tiga kelompok masing-masing 30 subyek, dimana setiap kelompok dilakukan pemeriksaan netrofil saliva dengan metode flowcytometry dan apus saliva. Kemudian di analisa kuantitas dan kualitas netrofil. Data hasil penelitian diuji dengan uji Anova/ Kruskal-wallis dan uji Chi- square. Hasil: Data penelitian didapatkan perbedaan bermakna (p < 0,050) jumlah netrofil saliva antara SIRS dengan kelompok normal/sehat, dan kelompok sepsis dengan kelompok normal/sehat, sedangkan untuk kelompok SIRS tidak terdapat perbedaan bermakna (p > 0,050) dengan kelompok sepsis. Aktivitas fagosit netrofil berbeda bermakna dari ketiga group (p < 0,050) Kesimpulan: Terdapat perbedaan jumlah dan kualitas netrofil saliva pada pasien yang mengalami SIRS dan sepsis dibandingkan dengan orang normal/sehat. Kata Kunci: Sepsis, Netrofil, Saliva, Flowcytometry.

    Item Type: Thesis (Masters)
    Subjects: Q Science > Q Science (General)
    Divisions: Pasca Sarjana > Magister
    Depositing User: Prima Adi Pradana
    Date Deposited: 19 Apr 2014 05:53
    Last Modified: 19 Apr 2014 05:53
    URI: https://eprints.uns.ac.id/id/eprint/12169

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