Pengembangan Produk Tempe Generasi Ketiga Berkhasiat Antioksidan Berbahan Baku Koro Benguk (Mucuna Pruriens L.D.C. Var. Utilis)

Ariani , Sri Retno Dwi and Handayani , Sri (2010) Pengembangan Produk Tempe Generasi Ketiga Berkhasiat Antioksidan Berbahan Baku Koro Benguk (Mucuna Pruriens L.D.C. Var. Utilis). HBL DIPA BLU APBN UNS. pp. 1-2.

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    Abstract

    The aim of this research is to develop the third generated of tempeh product from velvet beans (Mucuna Pruriens L.D.C. Var. Utilis) as the natural antioxidant for food product preserved. The samples are velvet beans from local farmer in Wonogiri Central Java. The Methods of this research are using literature studies and laboratory experiments. The procedures of this reasearch are : producing velvet beans tempeh, isolating and optimazing production of the third generated of tempeh product that has antioxidant activity from velvet beans, identify isoflavone (daidzein, genistein, glicytein and factor- 2) in product, implementing the product as food product preserved and stability test to product during stored in cold (40C), room temperature and heat temperatures (1500C, dan 1800C). The isoflavones were analysed by HPLC method dan antioxidant activities test by DPPH method. From the research results can be concluded that: (1) the most appropriate method to produce a third generation ektstract tempeh made from raw velvet bean potent antioxidant are: manufacture of velvet bean tempeh with chopped grain size with the addition of tempeh inoculum brand "RAPRIMA" containing Rhizopus oligosporus NRRL 2710, a long time of fermentation for 2 days, using the method of extraction solvent type B with 70% ethanol. The product was subsequently called velvet bean ethanol extract of tempeh (EETKB), (2) From EETKB production method above, each 100 grams tempeh produced EETKB velvet bean weighing 3794 mg, have antioxidant activity of 87.65% ± 0:12, containing 4 types of isoflavones with respective levels were: factor-2 = 0.88%, daidzein = 5.93%, glisitein = 3.63% and genistein = 7.80%, (3) Treatment EETKB addition of substrate types of oil (sample = VCO) with a long storage time of 3 days showed The best antioxidant activity, (4) Treatment with the addition EETKB 200 ppm concentration of VCO with storage at room temperature for 72 hours (3 days) showed the best antioxidant activity (DPPH value = 35.64%), (5) additional treatment EETKB 200 ppm of the VCO with a cold storage temperature (4oC) showed that storage time is longer than the room temperature is over 108 hours (4.5 days) (DPPH value = 35.31%), (6) Treatment EETKB adding 200 ppm of the VCO with heating treatment (150oC ) shows a relatively stable DPPH values with the highest antioxidant activity at 10 min heating (DPPH value = 27.46%), and (7) Treatment EETKB adding 200 ppm of the VCO with heating treatment (180oC) showed antioxidant activity is not stable and very low with values the highest DPPH on heating for 20 minutes (DPPH value = 7.4%).

    Item Type: Article
    Subjects: Q Science > Q Science (General)
    T Technology > TX Home economics
    Divisions: Lembaga Penelitian dan Pengabdian Kepada Masyarakat - LPPM
    Depositing User: Lia Primadani
    Date Deposited: 18 Apr 2014 21:39
    Last Modified: 18 Apr 2014 21:39
    URI: https://eprints.uns.ac.id/id/eprint/12044

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