IMMOBILISATION CURCUMIN ON CARBON PASTE ELECTRODE AS MODIFIER BIOSENSOR TO DETERMINATE URIC ACID

Hastuti, Sri and Martini, Tri and Kuncaka, Agus and , Nuryono (2010) IMMOBILISATION CURCUMIN ON CARBON PASTE ELECTRODE AS MODIFIER BIOSENSOR TO DETERMINATE URIC ACID. Hibah Pekerti Dikti. pp. 1-2.

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    Abstract

    Research about creating of carbon paste electrode modified by curcumin for detecting of uric acid has been conducted. Causing improperly condition, the research has been changed with another method, so the focus of research is “Immobilizations of HRP-Uricase Enzyme on Silica Material based on Sol-Gel as Biosensor for detecting of uric acid”. Goal of this research is study of silicamaterial useful (aquagel) for development of HRP-Uricase enzyme as uric acid biosensor. Optimization of biosensor cover: optimization of wavelength, pH optimization of HRP-Uricase enzyme physiologies, and optimization of HRP-Uricase enzyme physiologies temperature. Character of biosensor cover: linier range, detection limit, reproducible and sensitivity. The result shows that condition of optimization can be achieved at 490 nm wavelengths, time respons for concentration of uric acid is more than 30 minutes, pH 7 and temperature 25 o C. Character of biosensor cover: linier range at 0 – 100 ppm concentration, and 1,98 ppm limit detection. The other hand the reproducible is not improperly and its sensitivity is 0,002 linier range.

    Item Type: Article
    Subjects: R Medicine > R Medicine (General)
    R Medicine > RS Pharmacy and materia medica
    Divisions: Lembaga Penelitian dan Pengabdian Kepada Masyarakat - LPPM
    Depositing User: Lia Primadani
    Date Deposited: 18 Apr 2014 00:44
    Last Modified: 18 Apr 2014 00:44
    URI: https://eprints.uns.ac.id/id/eprint/11855

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