Cloning and Molecular Analysis of Hepatitis C Virus (HCV) 1a NS5B from HCV Isolated in Surakarta Indonesia

Prasetyo, Afiono Agung (2014) Cloning and Molecular Analysis of Hepatitis C Virus (HCV) 1a NS5B from HCV Isolated in Surakarta Indonesia. Journal of Biotechnology and BiodiversityJournal of Biotechnology and Biodiversity (2). pp. 10-18. ISSN 2087-0183

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    Abstract

    The hepatitis C virus (HCV) NS5B protein is known essential for virus replication and suggested play important role in therapy resistance mechanism. To predict and more understanding the molecular pattern of HCV 1a NS5B we cloned the NS5B gene of HCV 1a isolated in Central of Java Indonesia using stan-dard cloning procedure. The sequence results were then aligned with all of HCV 1a NS5B complete coding sequences reported in GenBank. In total, 397 se-quences were retrieved and aligned by ClustalW. We revealed the amino acid se-quence consensus of HCV NS5A gene. Overall, variations were found at aa 5, 11, 15, 33, 36, 37, 46, 47, 50, 55, 57, 59, 62, 63, 65, 66, 68, 71, 73, 77, 81, 82, 84-86, 90, 98, 101, 110, 113, 116, 117, 120, 124, 130, 134, 135, 144, 147-149, 151, 156, 162, 166, 173, 177-180, 184, 189, 198, 202, 206, 207, 209, 210, 212, 213, 231, 235, 238, 248, 251, 252, 254, 262, 267, 270, 273, 300, 307, 309, 310, 327, 330, 333, 334, 355, 374, 376, 377, 379, 389, 392, 400, 401, 402, 405, 412, 415, 421, 423-427, 431, 432, 434, 435, 437, 440, 451, 452, 454, 455, 459, 461, 464, 480, 487, 499, 506, 510, 512, 513,517, 520, 523, 531, 535, 540, 543, 544, 549, 552, 556, 564-566, 572, 574, 580, 581, 585, and 588. Amino acid variations may have relevant changes of physicochemical properties so that influence the repli-cation efficiency, therefore, the amino acid variations found in the present re-port need further study. Key words: HCV 1a, NS5B

    Item Type: Article
    Subjects: R Medicine > R Medicine (General)
    Divisions: Fakultas Kedokteran
    Depositing User: Devi Arlina Irawati
    Date Deposited: 16 Apr 2014 16:28
    Last Modified: 28 Oct 2016 15:50
    URI: https://eprints.uns.ac.id/id/eprint/11459

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