Mutation of the first ATG ORF-3 of Chicken Anemia Virus into ACG completely abolish the apoptin production

Prasetyo, Afiono Agung and Kamahora, Toshio (2014) Mutation of the first ATG ORF-3 of Chicken Anemia Virus into ACG completely abolish the apoptin production. Journal of Biotechnology and Biodiversity, 1 (1). pp. 3-7. ISSN 2087-0183

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    Abstract

    To test the effect of one point mutation on the first initiation codon of Chicken Anemia Virus (CAV) open reading frame-3 (ORF-3), an apoptin knocked out expressor plasmid pCLS-VP3(-) and a CAV apoptin knocked out plasmid pCAV/Ap(-) were constructed. In both plasmids, the first ATG in ORF-3 was mutated into ACG by a reversed long PCR. No apoptin detected in COS-1 cells transfected with pCLS-VP3(-) using western blotting and immunofluorescence assay, while apoptin was detected in COS-1 cells transfected with pCLS wild type. After released from pCAV/Ap(-), the complete genome of CAV/Ap(-) was ligated to form the replicative form. The apoptin production was completely abolished in MDCC-MSB1 cells transfected with replicative form of CAV/Ap(-). The apoptin production was fully regained after a reverse mutation into CAV/Ap(-)RM. These data shows the first evidence that mutation of the first ATG of ORF-3 into ACG could completely abolish the production of apoptin. Key words: Chicken Anemia Virus, Circovirus, Gyrovirus, Apoptin

    Item Type: Article
    Subjects: Q Science > QR Microbiology > QR355 Virology
    R Medicine > R Medicine (General)
    Divisions: Fakultas Kedokteran
    Depositing User: Devi Arlina Irawati
    Date Deposited: 15 Apr 2014 13:29
    Last Modified: 28 Oct 2016 15:52
    URI: https://eprints.uns.ac.id/id/eprint/11129

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