Morphology and RAPD (random amplification of polymorphic DNA) based classification of genetic variability of Java Salacca (Salacca zalacca Gaertner. Voss)

Nandariyah, (2014) Morphology and RAPD (random amplification of polymorphic DNA) based classification of genetic variability of Java Salacca (Salacca zalacca Gaertner. Voss). Journal of Biotechnology and Biodiversity, 1 (1). pp. 8-13. ISSN 2087-0183

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    Abstract

    The research aimed to role out the genetic variability and the classification of Java Salacca based on its morphological and molecular characters and to find out the genetic relationship among salacca cultivars that can be selected as parental material for breeding program. The salacca from Manonjaya (West Java), Banjarnegara, Bejalen, Lawu and Saratan (Central Java), Super Pondoh, Black Pondoh, Gading, Kembangarum, Madu and Manggala (Sleman-Yogyakarta) and Suwaru (East Java) were used in our study. Morphological characters and classification analysis by RAPD method with six random primers (OPA-11, OPA-16, OPA-17, OPA-18, OPX-15 and OPX-17) were used as classification variable. The genetic variability among cultivars of Java Salacca was presented by the similarity matrixes and dendogram. Based on the morphological classification, the twelve salacca cultivars was divided at four clusters: 1) Manonjaya, Manggala, Suwaru and Kembangarum, 2) Super Pondoh and Black Pondoh 3) Banjarnegara, Saratan and Bejalen, 4) Gading, Madu and Lawu. Based on the molecular-RAPD method, the twelve salacca cultivars was also divided into four clusters but difference member of cultivars: 1) Super Pondoh, Banjarnegara, Black Pondoh, Gading, Kembangarum and Suwaru, 2) Bejalen, Saratan and Lawu, 3) Madu and Manggala and 4) Manonjaya. Based on the two classification system used in the study I found the close relationship of Saratan Salacca and Bejalen Salacca from Central Java origin also Super Pondoh Salacca and Black Pondoh Salacca from Sleman-Yogyakarta origin. Key words: Morphology, Classification, Genetic variability, RAPD

    Item Type: Article
    Subjects: S Agriculture > S Agriculture (General)
    Divisions: Fakultas Pertanian > Agribisnis
    Depositing User: Devi Arlina Irawati
    Date Deposited: 15 Apr 2014 13:22
    Last Modified: 17 Mar 2017 14:43
    URI: https://eprints.uns.ac.id/id/eprint/11125

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