IDENTIFIKASI MOLEKULER KERAGAMAN TANAMAN MABAI (Pongamia pinnata) DAN PENGEMBANGAN TEKNOLOGI PERBANYAKAN IN VITRO UNTUK PENYEDIAAN BAHAN BAKU BIOFUEL MOLECULER IDENTIFICATION of Pongamia pinnata VARIABILITY for BIOFUELS MATERIAL SUPPLY

Yunus, Ahmad and , Samanhudi and T, Amalia and Rahayu, Muji (2010) IDENTIFIKASI MOLEKULER KERAGAMAN TANAMAN MABAI (Pongamia pinnata) DAN PENGEMBANGAN TEKNOLOGI PERBANYAKAN IN VITRO UNTUK PENYEDIAAN BAHAN BAKU BIOFUEL MOLECULER IDENTIFICATION of Pongamia pinnata VARIABILITY for BIOFUELS MATERIAL SUPPLY. Hibah Bersaing.

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    Abstract

    Exploration of biofuel sources from plant need developed for supply of alternative energy. Information of genetic diversity in Pongamia pinnata is necessary to support breeding programs and efforts conservation. Inter Simple Sequence Repeats (ISSR) marker could enhance the efficiency of early selecti on stage and reduce time consuming in P. Pinnata breeding. The research aims to get the kind of P. pinnata genetic information by moleculer using ISSR method. Field research was conducted in the Java, Sumatra, Sulawesi and Maluku. Laboratory research w as conducted on Plant Physiology and Agricultural Biotechnology Laboratory Faculty of Agriculture, Central Laboratory UNS Surakarta and Study Center of Tropic Fruit Laboratory, IPB. The study was conducted from April – October 2010. Sampling is taken ra ndomly in Java, Sumatra, Sulawesi and Maluku. Sixteen accession were amplified by six ISSR primers, i.e. PKBT2, PKBT3, PKBT4, PKBT6, PKBT7 dan PKBT9. The result of ISSR analyzed by hierarchial cluster analysis of NTSYS version 2.10 with SAHN method. The final resuld show as dendogram. From the results can be concluded that (1) DNA amplification with sixteen accession use PKBT 2, PKBT3, PKBT4, PKBT6, PKBT7 , PKBT9 primers get 3 loci untuk 8 loci with size from 100 bp until 1500 bp. (2) Dendogram showed 16 a ccesion of P.pinnata have 73% similarities level and produced three groups, i.e. Group A were A1, A2, A3, P4 accesion, group B were A4, M3, B2, Y1, Y2, Y3, Y4 accesion and group C were P1, P2, P3, M1, M2 accesion

    Item Type: Article
    Subjects: Q Science > QK Botany
    S Agriculture > S Agriculture (General)
    Divisions: Lembaga Penelitian dan Pengabdian Kepada Masyarakat - LPPM
    Depositing User: Lia Primadani
    Date Deposited: 15 Apr 2014 03:23
    Last Modified: 15 Apr 2014 03:23
    URI: https://eprints.uns.ac.id/id/eprint/11075

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