Karakterisasi fenotip kultivar padi tahan dan rentan wereng coklat, Nilaparvata lugens Stål. (Hemiptera: Delphacidae)

ASTUTI, PUJI and Supriyadi, and Supriyono, (2012) Karakterisasi fenotip kultivar padi tahan dan rentan wereng coklat, Nilaparvata lugens Stål. (Hemiptera: Delphacidae). Jurnal Entomologi Indonesia, 9 (2). pp. 1-7.

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    Abstract

    The brown planthopper, Nilaparvata lugens Stål. (Hemiptera: Delphacidae) is a destructive and widespread insect pest throughout the rice areas in Indonesia. Rice plant resistance is an effective method in controlling the brown plant hopper. However, the resistance sources and how their mechanism for imparting induced resistance against brown planthopper remains to be understood. This research was aimed to identify plant hair and protein banding patterns, as a genetic expression, of resistant and susceptible varieties. Identification of the variation of number of plant hair were carried out by using binocular microscope, while the protein banding patterns was detected by separation on SDS-Page. Co-variants analysis was adopted to identify the groups of resistant cultivars based on protein banding patterns. The results showed that plant hair on resistant rice cultivars are different with susceptible cultivars (resistance to Pelita I/1). The plant hair on resistant cultivars are larger/longer, more abudant and grew tighter than on susceptible cultivar (resistance to Pelita I/1). However the number of plant hair have no correlation with resistance level. In the absence of brown plant hopper infestation, the expression of protein total of resistant and susceptible cultivars are not clearly separated. The susceptible Pelita I/1 varieties showed a similar banding pattern to IR 26 (biotype 1), IR 42 (resistance to biotype 2) and IR IR 74 (resistance to biotype 3), but the four cultivars are not similar to the resistant cultivar IR 36 varieties (resistance to biotype 2). Our research showed that both plant hair and protein banding patterns can not be used to identify the plant resistance. Wereng coklat, Nilaparvata lugens Stål. (Hemiptera: Delphacidae) merupakan salah satu hama utama padi di Indonesia. Kultivar tahan wereng coklat telah dikembangkan guna menghadapi serangan hama tersebut. Akan tetapi, bentuk ekspresi ketahanan kultivar tahan terhadap wereng coklat masih belum dipahami dengan jelas. Penelitian ini dilakukan untuk mempelajari variasi rambut tanaman dan pola pita protein total pada kultivar tahan dan rentan terhadap wereng coklat. Identifikasi keberadaan rambut dilakukan secara langsung dengan mikroskop, sedangkan pola pita protein total dipelajari dengan teknik elektroforesis pada SDS-PAGE. Hasil penelitian menunjukkan bahwa rambut tanaman pada kultivar padi tahan wereng coklat berbeda dengan kultivar rentan (Pelita I/1). Rambut tanaman pada beberapa kultivar tahan, tumbuh lebih rapat, lebih banyak, dan atau lebih besar/panjang dibandingkan yang tumbuh relatif kultivar rentan (Pelita I/1). Namun, jumlah rambut tersebut tidak berkolerasi dengan tingkat ketahanan. Pada kedaan tidak diinfestasi wereng coklat, ekspresi pola pita protein total, antara kultivar rentan dan tahan, tidak berbeda secara nyata. Pelita I/1 (rentan) menunjukkan kemiripan kuat dengan IR 26 (tahan biotipe 1) dan menunjukkan kemiripan dengan IR 42 (tahan biotipe-2) dan IR 74 (tahan biotipe 3), namun keempat kultivar tersebut menunjukkan kemiripan lemah dengan kultivar tahan IR 36 (tahan biotipe 2). Penelitian ini menunjukkan bahwa baik rambut tanaman maupun pola pita protein total belum dapat digunakan untuk identifikasi varietas rentan dan tahan wereng coklat.

    Item Type: Article
    Subjects: S Agriculture > S Agriculture (General)
    Divisions: Pasca Sarjana > Magister > Agronomi
    Depositing User: Arissa Aprilia Nurcahyani
    Date Deposited: 15 Apr 2014 01:17
    Last Modified: 15 Apr 2014 01:17
    URI: https://eprints.uns.ac.id/id/eprint/11017

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