Isolation and Characterization of Phytase-Producing Bacteria from Extreme Region in Indonesia

Sajidan, Nuhriawangsa and Patriadi, Adi Magna and Adi , Ratriyanto (2010) Isolation and Characterization of Phytase-Producing Bacteria from Extreme Region in Indonesia. IbM.

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    Abstract

    Phytase is a phosphatase enzyme and has the ability to hydrolyze phytate compounds (myo-inositol (1,2,3,4,5,6) hexakisphosphates) to myo-inositol and inorganic P. There are several evidence that phytase enzyme could optimize the use of P in animal diet, reduce environmental pollution caused by inorganic P, thus prevent eutrofication on water surfaces. Only few of phytases from bacterial strains have been isolated, cloned, sequenced and expressed, for example Escherichia coli, Bacillus sp, Selenomonas ruminantium and Klebsiellapneumoniae. The objective of this research was to obtain characterized stable native bacteria "against high temperature and acidic pH conditions. The samples of the bacteria were isolated from soil and water obtained from the extreme heat areas in Indonesia such as upland Merapi Volcano, Yogyakarta and from hot spring in Gedongsongo, Ungaran. The samples of bacteria were mixed in a solution of 1.5 ml physiological NaCl 0.9%. Then, the bacteria were isolated in solid and liquid LB medium containing 0.4% phytic acid by incubation at a temperature of 37°C for 16 hours. The crude enzyme extract obtained from isolation process was centrifuged, and the supernatant was taken and tested thereafter for phytase activity in degrading phytate bond to myo-inositol and free phosphate. The enzyme was purified by Flow Liquid Protein Chromatography (FPLC) method and physicaly characterized including pH, optimal temperature and the influence of metal effectors on the relative enzyme activity. The isolation results indicated that volcanic soil obtained from Merapi Volcano contained bacteria including phytase producing bacteria. The selection of bacterial colonies indicated that bacterial colony from Merapi had the highest phytase activity. The characterization showed that phytase had an optimum at pH 5, optimum temperature at 40°C. The [S] relative activity was maximum on 2%. Moreover, phytase activity was catalyzed by Mg2+ (10-4M) but inhibited by Fe2+(l10-4M) ion. In conclusion, phytase producing bacteria can be isolated from volcanic materials such as soil and water obtained from upland Merapi volcano and hot spring Gedongsongo, Ungaran.

    Item Type: Article
    Subjects: Q Science > QH Natural history > QH301 Biology
    R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine
    Divisions: Lembaga Penelitian dan Pengabdian Kepada Masyarakat - LPPM
    Depositing User: Lia Primadani
    Date Deposited: 15 Apr 2014 00:59
    Last Modified: 15 Apr 2014 00:59
    URI: https://eprints.uns.ac.id/id/eprint/11016

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