IDENTIFIKASI MORFOLOGI DAN MOLEKULER SEBAGAI DASAR PENGEMBANGAN TANAMAN PORANG

Harjoko, Dwi and Sakya, Amalia T and Rahayu, Muji (2011) IDENTIFIKASI MORFOLOGI DAN MOLEKULER SEBAGAI DASAR PENGEMBANGAN TANAMAN PORANG. .

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    Abstract

    The research aims to get the kind of Amorphophallus oncophyllus having superior properties and produce high level of glucomannan and starch so appropriate to be cultivated based of morphological and molecular characters, get the most influence significant of agronomy variable on glucomannan and starch level. Field research was conducted in the Central Java and East Java. Laboratory research was conducted on Plant Physiology and Agricultural Biotechnology Laboratory Faculty of Agriculture and Central Laboratory UNS Surakarta. The study was conducted from April – October 2010. Sampling is taken randomly in four areas in Hutan Bromo Karanganyar, Jumantono, Saradan dan Nganjuk. Morphological identification is done by morphological observation involved observing performance, leaves, stems, roots flowers and tuber. Identification of molecular is done with RAPD method that is began with the isolation of DNA, the quality and quantity of DNA test, and DNA polymorphism analysis. Identification agronomist variable component is determined by soil conditions, micro climate and culture technique. Analysis og chemistry content involved glucomannan and starch. Data were analyzed using description analysis, and data from electrophoresis DNA were analysed based on the appearance RAPD band pattern of DNA using cluster analysis. From the results can be concluded that (1) Amorphophallus oncophyllus in the region Hutan Bromo Karanganyar, Jumantono, Saradan dan Nganjuk has same of the performance, leaves, stems, roots, flower and tuber; (2) Identification of molecular A. oncophyllus used three primer i.e. SBH 12, SBH 13, SBH 19 ; (3) Glucomannan and strach content are influenced total N, K exchange, Organic C, organic material, pH, C/N ratio, microclimat and culture technique. (4) Highest content of glucomannan and starch is A. oncophyllus from Saradan and lowest content of glucomannan and starch is A. oncophyllus from Hutan Bromo Karanganyar. Key word: A. oncophyllus, morphological, moleculer, glucomanan, strach Penelitian bertujuan mendapatkan jenis porang yang mempunyai sifat unggul dan menghasilkan kadar glukomanan dan pati tinggi sehingga sesuai untuk dibudidayakan berdasarkan karakter morfologi dan molekuler serta mendapatkan variabel agronomis yang paling berpengaruh terhadap pembentukan pati dan glukomanan. Penelitian lapangan dilaksanakan di Jawa Tengah dan Jawa Timur. Penelitian laboratorium dilakukan di Laboratorium Fisiologi Tanaman dan Bioteknologi Pertanian Fakultas Pertanian dan Laboratorium Sentral UNS Surakarta. Penelitian dilaksanakan mulai bulan April - Oktober 2010. Pengambilan sampel dilakukan secara acak di empat wilayah yaitu Hutan Bromo Karanganyar, Jumantono, Saradan dan Nganjuk. Identifikasi morfologi meliputi habitus, daun, batang, akar, bunga dan umbi. Identifikasi molekuler dimulai dengan isolasi DNA, kualitas dan kuantitas tes DNA, dan analisis polimorfisme DNA. Identifikasi komponen variabel agronomis ditentukan oleh kondisi tanah, iklim mikro dan tehnik budidaya yang diterapkan. Analisis kandungan kimia umbi porang meliputi glukomanan dan pati. Data dianalisis menggunakan analisis deskripsi, sedangkan data elektroforesis DNA dianalisis berdasarkan pola DNA dengan menggunakan cluster analysis. Dari Hasil penelitian dapat disimpulkan bahwa (1) Tanaman porang di daerah Hutan Bromo Karanganyar, Jumantono, Saradan dan Nganjuk mempunyai bentuk habitus, daun, batang, akar dan warna umbi yang sama, (2) Identifikasi molekuler untuk tanaman porang menggunakan 3 primer yaitu SBH 12, SBH 13, SBH 19; (3) Kandungan glukomanan dan pati umbi porang dipengaruhi oleh kondisi lingkungan baik tanah (N total, K tertukar, C organik, bahan organik, pH dan C/N rasio), iklim mikro dan tehnik budidaya. (4) Kandungan glukomanan dan pati tertinggi terdapat pada umbi porang dari Saradan, sedangkan terendah dari daerah Hutan Bromo Karanganyar. Kata kunci: porang, morfologi, molekuler, glukomanan, pati

    Item Type: Article
    Subjects: S Agriculture > S Agriculture (General)
    Divisions: Fakultas Pertanian
    Depositing User: Miranda Nur Q. A.
    Date Deposited: 14 Apr 2014 23:08
    Last Modified: 03 May 2014 22:39
    URI: https://eprints.uns.ac.id/id/eprint/10925

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